Recombinant Bacillus subtilis Uncharacterized protein ycbP (ycbP)

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Cat. No.

BT2059225

Source

in vitro E.coli expression system

Synonyms

ycbP; BSU02590; Uncharacterized protein YcbP; ORF15

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Description

Lack of Specific Data on ycbP

The search results focus on well-characterized B. subtilis recombinant proteins and expression systems, including:

YITB (Source ): A recombinant protein with a known sequence (MNETITYDTWNDMLSKQITDQLIDELDVLKWAYRTYGEKIVYACSFGAEGMVLLDLISKINKNAHIIFLDTGLHFQETYELIETVKERYPGFAIQMLEPELSLTEQGTKYGGELWKHNPNLCCQLRKIEPLKKHLSGMTAWISGLRRDQSPTRKHIQYVNLDQKFELIKICPLIHWTWDDVWTYIRLHNLPYNKLHDQHYPSIGCEMCTLPSPDPNDERAGRWAGREKTECGLHQE).
YjbA (Source ): A ClpC adaptor protein involved in sporulation, with structural insights from co-crystal studies.
Pylb (Source ): A stationary-phase promoter driving high expression of target genes (e.g., egfp, mApple).

No studies explicitly mention ycbP, its function, or its recombinant production.

Potential Confusion with Related Proteins

The term "ycbP" may overlap with other B. subtilis genes, such as:

Gene/Protein	Function/Role	Expression Context
YlbP	Stationary-phase gene; part of promoter selection for recombinant expression	Driven by Pylb promoter in B. subtilis
YddF	Stress-response protein; candidate for promoter-driven expression	High β-galactosidase activity in stationary phase
YITB	Uncharacterized protein (OPCA179865); available as recombinant protein	>85% purity via SDS-PAGE

**General Insights into B. subtilis Recombinant Protein Production

While ycbP-specific data is absent, B. subtilis remains a robust platform for recombinant protein production due to:

**Key Features of B. subtilis Expression Systems**

Advantage	Mechanism	Example
Secretion Efficiency	Signal peptides (e.g., CotG) direct proteins to extracellular medium	Rabies virus G protein expressed via CotG fusion (Source )
Inducible Promoters	Pylb, P43, and IPTG-regulated systems enable controlled expression	Pylb drives 7.8–11.3× higher expression than P43 (Source )
Protease Regulation	Chaperones (e.g., PrsA) prevent degradation; ClpC/YjbA systems manage substrate turnover	YjbA recruits ClpCP for spore maturation (Source )

Recommendations for Further Research

To address the gap in ycbP data:

Verify Nomenclature: Cross-check NCBI Gene ID or UniProt entries for ycbP.
Explore Homologs: Screen B. subtilis genome databases for proteins with similar sequences to ycbP.
Recombinant Production: Use established B. subtilis systems (e.g., Pylb promoter, CotG secretion) to express ycbP if cloning is feasible.

Product Specs

Form

Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.

Lead Time

Delivery times vary depending on the purchase method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and serves as a guideline.

Shelf Life

Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms maintain stability for 12 months at -20°C/-80°C.

Storage Condition

Upon receipt, store at -20°C/-80°C. Aliquot to prevent repeated freeze-thaw cycles.

Tag Info

Tag type is determined during the manufacturing process.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

ycbP; BSU02590; Uncharacterized protein YcbP; ORF15

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-128

Protein Length

full length protein

Species

Bacillus subtilis (strain 168)

Target Names

ycbP

Target Protein Sequence

MTHVKALAIKGIMTIIVLYLVLGLGFGFTFGDTLLMTIVLGVISYLLGDLYVLPKWNNMI ATLADFGLAFLVIWLMGMPLSMGMTGGEVALAALFGAIVMAIGEYCFHFYMMSKEIGKKH YLETRTDS

Uniprot No.

P42248

Target Background

Database Links

KEGG: bsu:BSU02590

STRING: 224308.Bsubs1_010100001433

Subcellular Location

Cell membrane; Multi-pass membrane protein.

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Recombinant Bacillus subtilis Uncharacterized protein ycbP (ycbP)

Description

Lack of Specific Data on ycbP

Potential Confusion with Related Proteins

**General Insights into B. subtilis Recombinant Protein Production

**Key Features of B. subtilis Expression Systems**

Recommendations for Further Research

Product Specs

Target Background

Quick Inquiry

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