Recombinant Bacillus subtilis UPF0703 protein ycgQ (ycgQ)
Description
Definition and Basic Characteristics
The ycgQ protein is encoded by the ycgQ gene (Uniprot ID: P94394) in Bacillus subtilis, annotated as a hypothetical protein (BSU03240). Its recombinant form is produced using bacterial expression systems, often with modifications for purification and stability. Key features include:
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Gene Name: ycgQ
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Protein Length: Full-length sequence spans 285 amino acids (1–285aa) .
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Host Systems: Expressed in E. coli, yeast, baculovirus systems, or cell-free platforms .
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Tagging: Frequently N-terminal His-tagged for affinity chromatography .
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Purity: Achieves >85% to >90% purity via SDS-PAGE validation .
Recombinant Production and Expression Systems
While Bacillus subtilis is a model organism for recombinant protein secretion (e.g., Sec and Tat pathways) , ycgQ is predominantly expressed in E. coli due to its simpler genetic manipulation and high yield. Key production strategies include:
Host Organisms and Tags
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Hosts: E. coli (most common), yeast, baculovirus, or mammalian cells .
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Tagging: His-tag for purification; no reported use of signal peptides for secretion in ycgQ .
Purification and Quality Control
Applications in Research
The recombinant ycgQ protein serves as a tool in biochemical and immunological studies:
Secretion Pathways in B. subtilis
Genetic Engineering Advances
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them when placing your order, and we will fulfill your request.
Note: All proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: bsu:BSU03240
STRING: 224308.Bsubs1_010100001808
