Recombinant Bacillus thuringiensis subsp. konkukian Protein CrcB homolog 1 (crcB1)
Description
Overview of CrcB1
CrcB1 (UniProt ID: Q6HBI3) is a recombinant protein derived from Bacillus thuringiensis subsp. konkukian, a strain known for its entomopathogenic properties. The protein is expressed as a full-length construct (1–137 amino acids) fused to an N-terminal His tag in Escherichia coli . Its primary sequence includes conserved regions associated with cysteine-rich metal-binding domains, suggesting potential roles in stress response or ion homeostasis.
Production and Purification
The recombinant protein is produced via heterologous expression in E. coli, followed by affinity chromatography using the His tag. Key quality control measures include:
Research Applications
CrcB1 is primarily utilized as an antigen in immunoassays, such as ELISA, due to its recombinant purity and specificity . Its potential roles in bacterial stress response mechanisms could align it with studies on:
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Metal ion homeostasis in Bacillus species.
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Cross-species homolog functionality (e.g., E. coli CrcB comparisons).
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Development of stress-resistant microbial strains for industrial use.
Cross-Referencing with Related Proteins
While CrcB1 itself is understudied, insights from analogous proteins in B. thuringiensis highlight broader trends:
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Cry Toxins: Unlike Cry proteins (e.g., Cry1Ac), which are insecticidal crystal toxins regulated by sporulation-specific sigma factors (ς<sup>E</sup>/ς<sup>K</sup>) , CrcB1 lacks direct evidence of pesticidal activity.
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Sporulation Linkage: Genes like cry1Ac and cry2Aa are transcribed during sporulation , but CrcB1’s expression timing remains uncharacterized.
Knowledge Gaps and Future Directions
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Functional Characterization: No studies directly link CrcB1 to stress response pathways or metal ion binding.
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Structural Studies: The protein’s 3D structure and cysteine-mediated interactions remain unresolved.
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Biotechnological Potential: Engineered variants could explore roles in microbial stress tolerance or bioremediation.
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them in your order notes, and we will accommodate your request.
Note: All our proteins are shipped with standard blue ice packs by default. If dry ice shipping is required, please inform us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: btk:BT9727_4784
