Recombinant Bacillus thuringiensis subsp. konkukian UPF0473 protein BT9727_4118 (BT9727_4118)
Description
Taxonomic and Genomic Context
Bacillus thuringiensis subsp. konkukian (strain 97-27) harbors a genome with numerous uncharacterized open reading frames (ORFs), including BT9727_4118. This protein is designated under the "UPF" (Uncharacterized Protein Family) classification, indicating its functional role remains under investigation. Like other UPF proteins in this strain (e.g., BT9727_2423 and BT9727_1129), BT9727_4118 likely encodes a small, secreted, or membrane-associated protein, potentially involved in sporulation, toxin production, or environmental adaptation .
Expression and Purification
Recombinant production of BT9727_4118 would follow protocols established for related proteins:
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Cloning: Likely expressed in E. coli BL21 or B. thuringiensis BMB171 using plasmid vectors (e.g., pHT3101) .
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Purification: Affinity chromatography (Ni-NTA for His-tagged proteins) followed by size-exclusion chromatography .
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Stability: Requires storage at -20°C in glycerol-containing buffers to prevent aggregation .
Research Gaps and Future Directions
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Functional Characterization: Ligand-binding assays, knockout studies, and proteomic profiling are needed to elucidate its role.
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Structural Analysis: X-ray crystallography or cryo-EM could resolve its 3D structure and identify catalytic/regulatory domains.
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Synergistic Potential: Co-expression with Cry/Vip toxins to assess enhancement of insecticidal activity .
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is specifically requested and agreed upon in advance. Additional fees apply for dry ice shipping.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
