Recombinant Bacillus weihenstephanensis Thymidine kinase (tdk)

Shipped with Ice Packs
In Stock
Cat. No.
BT32472
Source
Yeast
Synonyms
tdk; BcerKBAB4_5128; Thymidine kinase; EC 2.7.1.21
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT32472
Source
E.coli
Synonyms
tdk; BcerKBAB4_5128; Thymidine kinase; EC 2.7.1.21
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT32472
Source
E.coli
Synonyms
tdk; BcerKBAB4_5128; Thymidine kinase; EC 2.7.1.21
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT32472
Source
Baculovirus
Synonyms
tdk; BcerKBAB4_5128; Thymidine kinase; EC 2.7.1.21
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT32472
Source
Mammalian cell
Synonyms
tdk; BcerKBAB4_5128; Thymidine kinase; EC 2.7.1.21
Purity
>85% (SDS-PAGE)
Quick Inquiry

Description

Introduction to Recombinant Bacillus weihenstephanensis Thymidine Kinase (tdk)

Thymidine kinase (tdk) is a critical enzyme in nucleotide metabolism, catalyzing the phosphorylation of thymidine to thymidine monophosphate (dTMP) via the salvage pathway. In Bacillus weihenstephanensis, a psychrotolerant member of the Bacillus cereus group, this enzyme plays roles in DNA synthesis and repair, particularly under stress conditions. Recombinant forms of this enzyme are engineered to study its biochemical properties, optimize industrial applications, or develop biotechnological tools .

Genetic and Molecular Characterization

The tdk gene in B. weihenstephanensis is part of a conserved genomic region shared with closely related species like B. cereus and B. anthracis. Key features include:

PropertyDetails
Gene Length~600–700 bp (varies by strain)
Protein Length~200 amino acids
Molecular Weight~22–24 kDa
Conserved DomainsATP-binding site, thymidine-binding pocket, catalytic loop
Homology85–90% sequence similarity with B. cereus TK

Recombinant tdk is typically expressed in Escherichia coli systems for high-yield production, leveraging vectors like pET or pQE with N-terminal His-tags for purification .

Enzymatic Activity

  • Substrate Specificity: Phosphorylates thymidine and select nucleoside analogs (e.g., azidothymidine) .

  • Kinetic Parameters:

    • KmK_m (thymidine): ~5–10 μM (similar to B. anthracis TK) .

    • Optimal pH: 7.0–8.0; retains activity at low temperatures (8–15°C) .

Thymidine Production

Strain engineering strategies in Bacillus spp. often involve tdk deletion to block thymidine salvage pathways, forcing reliance on de novo synthesis. Recombinant tdk can restore this pathway or modulate metabolic flux .

Drug Activation

Like other bacterial TKs, recombinant B. weihenstephanensis tdk can phosphorylate antiviral prodrugs (e.g., acyclovir), though this application remains exploratory .

Expression and Purification

  • Expression System: E. coli BL21(DE3) with IPTG induction .

  • Purification: Nickel-affinity chromatography yields >90% purity .

Table 1: Recombinant tdk Production Metrics

ParameterValueSource
Yield10–15 mg/L culture
StabilityStable at -80°C in Tris-PBS + 6% trehalose
Activity Retention>80% after 6 months

Stress Response Linkage

  • tdk expression correlates with acid resistance in B. weihenstephanensis, suggesting regulatory cross-talk between nucleotide metabolism and stress adaptation .

  • Transcriptional profiling identifies tdk as part of the lexA regulon, activated under DNA damage .

Challenges and Future Directions

  • Thermostability: Native B. weihenstephanensis tdk is psychrotolerant, limiting industrial use at higher temperatures .

  • Engineering Efforts: Site-directed mutagenesis to enhance activity or substrate range is ongoing .

Product Specs

Form
Lyophilized powder. We will ship the available format, but you can request a specific format when ordering.
Lead Time
Delivery times vary. Consult your local distributor for specifics. Proteins are shipped with blue ice packs. Request dry ice in advance (extra fees apply).
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please let us know and we will prioritize its development.
Synonyms
tdk; BcerKBAB4_5128; Thymidine kinase; EC 2.7.1.21
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-194
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Bacillus weihenstephanensis (strain KBAB4)
Target Names
tdk
Target Protein Sequence
MYLMNQNGWI EVICGSMFSG KSEELIRRIR RTQFAKQHAI VFKPCIDNRY SEEDVVSHNG LKVKAVPVSA SKDIFNHVTE EMDVIAIDEV QFFDGDIVEV VQVLANRGYR VIVAGLDQDF RGLPFGQVPQ LMAIAEHVTK LQAVCSECGS PASRTQRLID GEPAAFDDPI ILVGASESYE PRCRHCHAVP TKQR
Uniprot No.
A9VSC8

Target Background

Database Links

KEGG: bwe:BcerKBAB4_5128

STRING: 315730.BcerKBAB4_5128

Protein Families
Thymidine kinase family
Subcellular Location
Cytoplasm.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2025 TheBiotek. All Rights Reserved.