Recombinant Bacteroides thetaiotaomicron 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50% and may serve as a guideline.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and the protein's inherent stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If a specific tag type is required, please inform us for preferential development.
The tag type is determined during production. If a specific tag type is required, please inform us for preferential development.
Synonyms
ispD; BT_39232-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; EC 2.7.7.60; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-219
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Target Names
ispD
Target Protein Sequence
MKQSVIIVAG GKGLRMGSDL PKQFLPVGGK PVLMHTLEAF RKYDAMLQII LVLPREQQDF
WKQLCEEHHF SVEHLVADGG ETRFHSVKNG LALVQAPGLV GVHDGVRPFV TLEVIRRCYE
LAEQHKAVIP VVDVVETLRH LTDAGSETVS RTEYKLVQTP QVFEVELLKQ AYGQEFTPFF
TDDASVVEAM GVPVHLAEGN RENIKITTPF DLKIGSALL
Uniprot No.
Target Background
Function
Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).
Database Links
KEGG: bth:BT_3923
STRING: 226186.BT_3923
Protein Families
IspD/TarI cytidylyltransferase family, IspD subfamily
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