Recombinant Bacteroides thetaiotaomicron DNA replication and repair protein recF (recF)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we will prioritize shipping the format currently in stock, please specify your format preference in order notes for fulfillment.
Note: While we will prioritize shipping the format currently in stock, please specify your format preference in order notes for fulfillment.
Lead Time
Delivery times vary depending on the purchase method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, provided for your reference.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot to prevent repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type will be determined during the production process. If you require a specific tag, please inform us; we will prioritize its development.
The tag type will be determined during the production process. If you require a specific tag, please inform us; we will prioritize its development.
Synonyms
recF; BT_4255; DNA replication and repair protein RecF
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-369
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Target Names
recF
Target Protein Sequence
MILKRISILN YKNLEQVEIG FSAKLNCFFG QNGMGKTNLL DAVYFLSFCK SSGNPIDSQN
IRHEQDFFVI QGFYEAEDGT PEEIYCGMKR RSKKQFKRNK KEYSRFSDHI GFLPLVMVSP
ADSELIAGGS DERRRFMDVV ISQYDKEYLE ALIRYNKALA QRNTLLKSEF PVEEELFLVW
EEMMAQAGEI VFRKREAFIE EFIPIFQSFY SFISQDKEQV GLSYDSHARD ASLLEVLKQS
RERDKIMGFS LRGIHKDELN MLLGDFPIKK EGSQGQNKTY LVALKLAQFD FLKRTGQTVP
LLLLDDIFDK LDASRVEQIV KLVAGDNFGQ IFITDTNREH LDRILQKVGS DYKVFRVDQG
VINEMGAEQ
Uniprot No.
Target Background
Function
RecF protein is involved in DNA metabolism, essential for DNA replication and normal SOS response induction. It exhibits preferential binding to single-stranded, linear DNA and appears to also bind ATP.
Database Links
KEGG: bth:BT_4255
STRING: 226186.BT_4255
Protein Families
RecF family
Subcellular Location
Cytoplasm.
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