Recombinant Bacteroides thetaiotaomicron Ribosomal RNA large subunit methyltransferase F (rlmF)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.
Lead Time
Delivery times vary depending on purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, provided as a reference.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If you require a specific tag, please inform us for preferential development.
Synonyms
rlmF; BT_4691; Ribosomal RNA large subunit methyltransferase F; EC 2.1.1.181; 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-307
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Target Names
rlmF
Target Protein Sequence
MAERSELHTR NKHNGQYDFS LLTENYPPLR KFVLLNPLGI QTIDFFNPHA VKALNKALLI
SYYGIRYWDI PRNYLCPPIP GRADYVHYIA DLIDPERVSN TANEENGDKP KRQCRCLDIG
VGANCIYPII GHVEYGWMFV GSDIDPVSIE NARKIVTCNP VLAHKIDLRL QKDNRRIFDG
IIAPDEYFDV TICNPPFHSS KKEAEEGTLR KLSSLKGEKV KKTKLNFGGN ANELWCEGGE
LRFLLNMISE SRKYRKNCGW FTSLVSKEKN LDKLYAKLKA VHVSEYKIIR MCQGTKNSRI
LAWRFLE
Uniprot No.
Target Background
Function
Specifically methylates adenine at position 1618 of 23S rRNA.
Database Links
KEGG: bth:BT_4691
STRING: 226186.BT_4691
Protein Families
Methyltransferase superfamily, METTL16/RlmF family
Subcellular Location
Cytoplasm.
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