Recombinant Balaenoptera musculus NADH-ubiquinone oxidoreductase chain 3 (MT-ND3)
Description
Definition and Basic Characteristics
Recombinant MT-ND3 refers to the Balaenoptera musculus (blue whale) NADH dehydrogenase subunit 3 protein synthesized using recombinant DNA technology. It is a hydrophobic transmembrane protein critical for the catalytic activity of mitochondrial complex I (NADH:ubiquinone oxidoreductase), which transfers electrons from NADH to ubiquinone while pumping protons across the mitochondrial inner membrane .
Expression and Purification
-
Produced in E. coli with an N-terminal His-tag for affinity chromatography .
-
Lyophilized for long-term storage, reconstituted in Tris/PBS buffer with glycerol .
Research Applications
-
Antibody Production: Used as an immunogen for generating polyclonal antibodies (e.g., ab204977) .
-
Enzyme Activity Studies: Investigates complex I dysfunction in mitochondrial diseases .
-
Structural Biology: Supports crystallography or cryo-EM studies of respiratory chain complexes .
Clinical and Pathogenic Relevance
-
Disease Associations: Mutations in MT-ND3 are linked to mitochondrial disorders such as Leigh syndrome (LS) and Leber’s hereditary optic neuropathy (LHON) . For example, the A47T substitution (10197G>A mutation) disrupts complex I assembly and reduces catalytic activity by ~70% .
-
Therapeutic Target: Recombinant ND3 enables functional rescue studies in cell models of mitochondrial disease .
Research Challenges
Product Specs
Note: While we will prioritize shipping the format currently in stock, we are happy to accommodate specific format requests. Please indicate your preferred format in the order notes, and we will do our best to fulfill your requirements.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please communicate this to us in advance as additional fees may apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
