Recombinant Bartonella henselae Isoleucine--tRNA ligase (ileS), partial

Overview of Recombinant Bartonella henselae Isoleucine-tRNA Ligase (ileS), Partial

Recombinant Bartonella henselae Isoleucine--tRNA ligase (ileS), partial, refers to a genetically engineered, incomplete form of the Isoleucine--tRNA ligase enzyme derived from the bacterium Bartonella henselae . Isoleucine--tRNA ligase (IleRS) is a crucial enzyme responsible for catalyzing the attachment of isoleucine to its corresponding transfer RNA (tRNA) . This process is essential for accurate protein synthesis within cells .

Function and Importance of Isoleucine-tRNA Ligase (ileS)

Isoleucine--tRNA Ligase (IleRS) ensures the correct translation of genetic code by specifically binding isoleucine and attaching it to tRNA molecules that recognize isoleucine codons . This enzyme plays a critical role in maintaining the fidelity of protein synthesis . IleRS prevents the misincorporation of similar amino acids like valine through pre- and post-transfer editing mechanisms .

Role in Bartonella henselae

Bartonella henselae is a bacterium known to cause cat-scratch disease in humans . The isoleucine--tRNA ligase (ileS) is vital for the bacterium's survival and pathogenicity . Studying this enzyme in B. henselae can provide insights into the bacterium's cellular mechanisms and potential targets for therapeutic interventions .

Recombinant Production

The recombinant form of B. henselae Isoleucine--tRNA ligase (ileS), partial, is produced using genetic engineering techniques . The gene encoding the enzyme is isolated, modified (in this case, to produce a partial sequence), and inserted into a host organism (e.g., E. coli) for expression . The resulting protein is then purified for research purposes . The "partial" designation indicates that the recombinant protein represents only a fragment of the full-length enzyme .

Applications in Research

Recombinant B. henselae Isoleucine--tRNA ligase (ileS), partial, has several applications in scientific research:

• Structural Studies: Partial protein sequences can be used to determine the three-dimensional structure of the enzyme, providing insights into its function and interactions .

• Drug Discovery: The enzyme can be used as a target for developing new drugs to inhibit bacterial growth .

• Enzyme Kinetics: Studying the activity of the recombinant enzyme can help elucidate its catalytic mechanisms .

• Antibiotic Resistance: Isoleucyl-tRNA synthetases are drug targets, and bacterial resistance often arises through alterations in the second clade of the enzyme, IleRS2 .