Recombinant Bartonella henselae Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp)
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Product Specs
Form
Lyophilized powder
Note: While we will prioritize shipping the format currently in stock, please specify your format preference in order remarks for customized fulfillment.
Note: While we will prioritize shipping the format currently in stock, please specify your format preference in order remarks for customized fulfillment.
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is specifically requested and agreed upon in advance. Additional fees will apply for dry ice shipping.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is specifically requested and agreed upon in advance. Additional fees will apply for dry ice shipping.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, which may serve as a guideline.
Shelf Life
Shelf life depends on several factors including storage conditions, buffer composition, temperature, and the inherent stability of the protein. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The specific tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
The specific tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
Synonyms
tsaD; gcp; BH16110tRNA N6-adenosine threonylcarbamoyltransferase; EC 2.3.1.234; N6-L-threonylcarbamoyladenine synthase; t(6)A synthase; t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD; tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-364
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1) (Rochalimaea henselae)
Target Names
tsaD
Target Protein Sequence
MRLLGIETSC DETATAVIEH TIGGNSQILS NIVWSQTDHH APYGGVVPEI AARAHVEILD
ELILKALRDA HTKLKDIDAI AVTNGPGLIG GLLVGVMSAK ALSLATGKPF IGVNHLEGHA
LTAVLTHNVH FPYLLLLVSG GHTQTILVHG VGNYQRLGTT IDDALGEAFD KTAKLLGLPY
PGGPAIEKAA LLGNKNRIPL PRPLKGEKRL DFSFSGLKTA VRQAATAISP LTESDVADIA
ASFQAAVTDT VYDRVYLALQ HFTQQYPLSR HQGPRPPALV VAGGVAANQA IRLTLQELAD
QQGFEFIAPP LSLCTDNAAM IAFAGAQRLA RGEKSSLDIA PRSRWPLDEK SAPLIGTGRR
GTKA
Uniprot No.
Target Background
Function
This protein is essential for the formation of a threonylcarbamoyl group at adenosine position 37 (t6A37) in tRNAs that recognize codons beginning with adenine. It participates in the transfer of the threonylcarbamoyl moiety from threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, in conjunction with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.
Database Links
KEGG: bhe:BH16110
STRING: 283166.BH16110
Protein Families
KAE1 / TsaD family
Subcellular Location
Cytoplasm.
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