Recombinant Bartonella quintana Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD)

Introduction to Recombinant Bartonella quintana Acetyl-Coenzyme A Carboxylase Carboxyl Transferase Subunit Beta (accD)

The compound Bartonella quintana Acetyl-Coenzyme A Carboxylase Carboxyl Transferase subunit beta (accD) is a protein component of the acetyl-CoA carboxylase (ACCase) enzyme complex found in the bacterium Bartonella quintana . ACCase is a crucial enzyme in fatty acid biosynthesis, catalyzing the ATP-dependent carboxylation of acetyl-CoA to form malonyl-CoA, which is a key building block for fatty acid synthesis .

Characteristics of Bartonella quintana

Bartonella quintana is a bacterium transmitted by body lice that can cause trench fever and other diseases in humans . It primarily affects populations experiencing homelessness or those with limited access to water . Understanding the metabolic pathways of B. quintana, including fatty acid synthesis, is important for developing potential therapeutic strategies against this pathogen .

Acetyl-CoA Carboxylase (ACCase) and its Subunits

ACCase is a multi-subunit enzyme complex . In bacteria, it typically consists of four subunits:

• biotin carboxylase (BC)

• biotin carboxyl carrier protein (BCCP)

• carboxyl transferase alpha subunit (CTα)

• carboxyl transferase beta subunit (CTβ or accD)

The carboxyl transferase (CT) component, composed of CTα and CTβ (accD), is responsible for the second step of the ACCase reaction, transferring the carboxyl group from carboxybiotin to acetyl-CoA, forming malonyl-CoA .

Function of AccD Subunit

The accD subunit, or carboxyl transferase subunit beta, plays a vital role in the carboxyl transferase activity of the ACCase complex . It works in conjunction with the CTα subunit to facilitate the transfer of the carboxyl group, which is essential for the synthesis of malonyl-CoA .

Recombinant Production and Study

Recombinant accD refers to the protein produced using genetic engineering techniques, where the gene encoding accD from Bartonella quintana is cloned and expressed in a host organism (e.g., E. coli) . This allows for the production of a large quantity of the protein for research purposes, such as studying its structure, function, and interactions with other molecules .

Potential Applications

Studying recombinant B. quintana accD can provide insights into the fatty acid metabolism of this bacterium . This knowledge can potentially be exploited for developing new antibacterial agents that target ACCase, thereby inhibiting fatty acid synthesis and bacterial growth . Additionally, recombinant accD can be used in diagnostic assays or as a target for antibody development .

Data Table: Characteristics of Recombinant Bartonella quintana AccD

Due to the limited information, the following table contains general information regarding the accD protein.