Recombinant Bartonella tribocorum Triosephosphate isomerase (tpiA)
Description
Production and Purification
Expression System
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Yield: Typically produced at 0.5 mg/mL in a Tris-HCl buffer (pH 8.0) with 10% glycerol and 1 mM DTT .
Purity and Quality Control
Table 1: Key Properties of Recombinant B. tribocorum tpiA
Functional Insights
Metabolic Role
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Central to glycolysis, ensuring efficient energy production in B. tribocorum .
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Knockout studies in related bacteria (e.g., Pseudomonas aeruginosa) show that tpiA disruption alters carbon metabolism, increases membrane potential, and enhances antibiotic susceptibility .
Potential Pathogenicity Factors
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TIMs in Bartonella spp. may indirectly influence virulence by modulating metabolic pathways critical for host colonization .
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Structural similarities to TIMs in other pathogens (e.g., Bartonella henselae) suggest conserved mechanisms in eukaryotic cell invasion .
Research Applications
Diagnostic Development
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Recombinant TIMs are used in serological assays (e.g., ELISA) to detect Bartonella infections, though cross-reactivity remains a challenge .
Drug Target Exploration
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TIM is a validated target in parasitic diseases due to its essential role in glycolysis .
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Unique surface-exposed cysteine residues in Bartonella TIMs (e.g., 9 cysteines per monomer in tick TIMs) could enable species-specific inhibitor design .
Table 2: TIM Features Across Species
Future Directions
Product Specs
Target Background
KEGG: btr:BT_0852
STRING: 382640.Btr_0852
