Recombinant Bdellovibrio bacteriovorus DNA-directed RNA polymerase subunit beta (rpoB), partial
Description
Molecular and Functional Overview
The rpoB gene in Bdellovibrio bacteriovorus encodes the β subunit of the DNA-directed RNA polymerase (RNAP), a critical enzyme for transcription. This subunit is part of the catalytic core (α₂ββ'ω) and plays roles in RNA synthesis initiation, elongation, and antibiotic resistance mechanisms . The recombinant partial form of this protein (accession: YP_007023858.1) corresponds to a truncated sequence of the full-length rpoB, produced via heterologous expression systems for structural or functional studies .
Key Features of the B. bacteriovorus rpoB Gene (Strain Tiberius):
| Feature | Details |
|---|---|
| Genome Accession | NC_019567 |
| Gene Position | 3,067,253 – 3,071,368 bp (complement strand) |
| DNA Sequence Length | 4,116 bp |
| Protein Length | Partial sequence (exact residues unspecified) |
| Putative Function | RNA polymerase activity, rifampicin binding, transcription regulation |
Common Mutations and Phenotypic Effects in Bacterial rpoB:
| Organism | Mutation Sites (Codon) | Resistance Level | Fitness Cost |
|---|---|---|---|
| Mycobacterium tuberculosis | 516, 526, 531 | High | Low |
| Staphylococcus aureus | 526 | High | Moderate |
| Escherichia coli | 507–533 (Cluster I) | Variable | High |
Recombinant Production and Applications
While no direct studies on recombinant B. bacteriovorus rpoB were identified in the provided sources, insights can be extrapolated from related systems:
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Cloning Strategies: The gene is likely inserted into vectors (e.g., pET or pGEX) under inducible promoters (e.g., T7 or lac) .
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Purification: Affinity tags (His-tag, GST) enable isolation via chromatography .
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Applications:
Comparative Genomic and Transcriptional Data
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Conservation: The rpoB gene is single-copy and highly conserved across Bdellovibrio strains (e.g., HD100, Tiberius) .
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Expression: RNA-seq data from B. bacteriovorus HD100 suggests phase-specific transcription patterns, though rpoB itself was not highlighted in the differential expression analysis .
Technical Challenges and Future Directions
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during production. If a specific tag type is required, please inform us, and we will prioritize its development.
Target Background
KEGG: bba:Bd2984
STRING: 264462.Bd2984
