Recombinant Bdellovibrio bacteriovorus Guanylate kinase (gmk)

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Cat. No.
BT48941
Source
Yeast
Synonyms
gmk; Bd1568Guanylate kinase; EC 2.7.4.8; GMP kinase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT48941
Source
E.coli
Synonyms
gmk; Bd1568Guanylate kinase; EC 2.7.4.8; GMP kinase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT48941
Source
E.coli
Synonyms
gmk; Bd1568Guanylate kinase; EC 2.7.4.8; GMP kinase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT48941
Source
Baculovirus
Synonyms
gmk; Bd1568Guanylate kinase; EC 2.7.4.8; GMP kinase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT48941
Source
Mammalian cell
Synonyms
gmk; Bd1568Guanylate kinase; EC 2.7.4.8; GMP kinase
Purity
>85% (SDS-PAGE)
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Description

Functional Role of Guanylate Kinase (GMK)

  • Firmicutes, Actinobacteria, and Deinococcus-Thermus: GMK is strongly inhibited by (p)ppGpp .

  • Proteobacteria: GMK is resistant to (p)ppGpp inhibition; instead, (p)ppGpp targets RNA polymerase (RNAP) .

B. bacteriovorus, a Deltaproteobacterium, likely exhibits GMK properties consistent with its phylogenetic class. Proteobacterial GMKs (e.g., Escherichia coli) are not inhibited by (p)ppGpp, suggesting similar resistance in B. bacteriovorus .

Recombinant GMK Production and Applications

While B. bacteriovorus GMK has not been explicitly characterized in recombinant form, genetic tools for heterologous expression in this predator are well-established:

  • Expression Systems:

    • Plasmids with RSF1010 origins replicate in B. bacteriovorus .

    • Chromosomal integration via Tn7 transposons enables stable gene expression .

  • Promoters: Constitutive (e.g., PJ23119) and inducible (e.g., PJ ExD/EliR) promoters have been validated for recombinant protein production .

Comparative Kinetic Properties of Bacterial GMKs

The table below summarizes kinetic parameters of GMKs from diverse bacteria :

Species/Phylumkcatk_{cat} (s⁻¹)KmK_m (μM, GMP)KiK_i (μM, pppGpp)Inhibition Mode
Bacillus subtilis23.0 ± 1.024.6 ± 3.413.5 ± 2.1Competitive
Escherichia coliNot inhibited--Resistant
Thermus thermophilus4.8 ± 0.417.5 ± 4.12.9 ± 0.5Competitive
Streptomyces coelicolor25.5 ± 0.729.7 ± 3.461.5 ± 9.6Competitive

Note: B. bacteriovorus GMK is hypothesized to align with Proteobacterial resistance to (p)ppGpp, though experimental validation is needed.

Research Gaps and Future Directions

  1. Enzyme Characterization: No direct studies on B. bacteriovorus GMK kinetics or structure exist. Recombinant expression systems could enable purification and functional assays.

  2. Regulatory Mechanisms: The role of (p)ppGpp in B. bacteriovorus metabolism remains unexplored. Comparative studies with Firmicutes GMKs may reveal evolutionary adaptations.

  3. Biotechnological Potential: Engineered B. bacteriovorus strains could leverage GMK modulation to enhance predatory efficiency or metabolic engineering .

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specific delivery information. All proteins are shipped with blue ice packs by default. Request dry ice shipment in advance (extra fees apply).
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us and we will prioritize its development.
Synonyms
gmk; Bd1568Guanylate kinase; EC 2.7.4.8; GMP kinase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-185
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Target Names
gmk
Target Protein Sequence
MKTRMIIVAA PSGAGKSSFV ERITREDSRL VDIVTFTTRS IRQGETPGLQ YNFIDHADFE QKIKEGFFVE WAKVHTNFYG TSYSSLETAW NQGKTAIMDI DIQGVATFKS KFPDAKTVFI HPPSIDELRR RIEKRDGKVP ADIEVRMANA EKEIREASKF DYQIVNDVFE KSYGEFKKIV EDLLA
Uniprot No.
P60549

Target Background

Function
Essential for recycling GMP and indirectly, cGMP.
Database Links

KEGG: bba:Bd1568

STRING: 264462.Bd1568

Protein Families
Guanylate kinase family
Subcellular Location
Cytoplasm.

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