Recombinant Bdellovibrio bacteriovorus Ribonuclease 3 (rnc)
Description
Introduction to Recombinant Bdellovibrio bacteriovorus Ribonuclease 3 (Rnc)
Recombinant Bdellovibrio bacteriovorus Ribonuclease 3 (Rnc) is an enzyme that belongs to the ribonuclease III family and is involved in the processing and maturation of RNA precursors into functional rRNA, mRNA, and other small RNAs . RNase III enzymes are double-strand-specific endoribonucleases that play a crucial role in gene expression regulation in bacteria .
Rnc in Bdellovibrio bacteriovorus
Bdellovibrio bacteriovorus is a predatory bacterium known for its ability to invade and consume other bacteria . Rnc in B. bacteriovorus contributes to its predatory lifestyle by:
-
Degradation of Host DNA During the predatory cycle, B. bacteriovorus secretes nucleases to degrade the host's DNA within the bdelloplast (the structure formed after invasion) .
-
Role in Host-Independent Growth Rnc is also expressed during the host-independent growth phase, suggesting its involvement in processing nucleic acids for nutrient acquisition or other metabolic processes .
Rnc and Antibiotic Resistance
Predatory bacteria like Bdellovibrio bacteriovorus offer a potential tool to combat antibiotic resistance . They can eliminate pathogens and reduce the pool of antibiotic resistance genes through the secretion of nucleases that degrade exogenous DNA .
Rnc in Salmonella enterica
-
Overexpression in Virulent Strains The rnc gene is overexpressed in high-virulence strains of Salmonella enterica .
-
Impact on Intracellular Survival Deletion of the rnc gene in Salmonella leads to reduced intracellular survival and replication rates in macrophage cells .
-
Modulation of Host Immune Response RNAs extracted from rnc knockout mutants induce higher levels of immune responses in the host, including innate immune factors like IFN-β, RIG-I, and MDA-5 . This is attributed to the accumulation of dsRNA in the absence of RNase III activity .
Methods for Studying Rnc
-
Immunoblotting Immunoblotting assays can be used to detect dsRNA accumulation in rnc knockout mutants .
-
Quantitative PCR (qRT-PCR) qRT-PCR is used to measure the expression levels of rnc and other genes throughout the bacterial life cycle .
-
DNase Assays DNase assays determine the extracellular DNase activity of bacterial strains with and without rnc .
-
RNase Activity Assays RNase activity assays assess the ability of RNC to degrade dsRNA .
Impact on Virulence
| Feature | Wild-Type Salmonella | Δrnc Mutant Salmonella |
|---|---|---|
| rnc Gene Expression | High | Low |
| Intracellular Survival | High | Low |
| dsRNA Levels | Low | High |
| Host Immune Response | Low | High |
| Production of SodA protein | High | Suppressed |
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
KEGG: bba:Bd1941
STRING: 264462.Bd1941
