Recombinant Bifidobacterium adolescentis Elongation factor Tu (tuf)

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Cat. No.

BT43497

Source

Yeast

Synonyms

tuf; BAD_0533Elongation factor Tu; EF-Tu

Purity

>85% (SDS-PAGE)

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Cat. No.

BT43497

Source

E.coli

Synonyms

tuf; BAD_0533Elongation factor Tu; EF-Tu

Purity

>85% (SDS-PAGE)

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Cat. No.

BT43497

Source

E.coli

Synonyms

tuf; BAD_0533Elongation factor Tu; EF-Tu

Purity

>85% (SDS-PAGE)

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Cat. No.

BT43497

Source

Baculovirus

Synonyms

tuf; BAD_0533Elongation factor Tu; EF-Tu

Purity

>85% (SDS-PAGE)

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Cat. No.

BT43497

Source

Mammalian cell

Synonyms

tuf; BAD_0533Elongation factor Tu; EF-Tu

Purity

>85% (SDS-PAGE)

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Description

Introduction to EF-Tu and Recombinant Production

Elongation Factor Tu (EF-Tu), encoded by the tuf gene, is a GTP-binding protein critical for bacterial translation. In Bifidobacterium adolescentis, EF-Tu facilitates aminoacyl-tRNA binding to ribosomes during protein synthesis . Recombinant EF-Tu refers to heterologous expression of this protein, often in Escherichia coli or other hosts, for structural, functional, or therapeutic studies .

**Key Features of B. adolescentis EF-Tu**

Genomic Context: B. adolescentis contains a single tuf gene, unlike some eubacteria with duplicated copies .
Operon Structure: In Bifidobacterium, tuf is part of a conserved operon distinct from Lactobacillus .
Expression Systems: Recombinant EF-Tu is produced via plasmid cloning (e.g., pQE-30 Xa, pBCMAT) and optimized for yield in E. coli .

Genomic Context and Expression Systems

The tuf gene in B. adolescentis is tightly regulated and evolutionarily conserved. Below are key findings from genomic and cloning studies:

Feature	Details	Sources
Gene Structure	Single copy in B. adolescentis genome; flanked by ribosomal protein genes
Operon Composition	Includes genes encoding ribosomal proteins (e.g., rplU, rpmB)
Cloning Primers	TUF-1/TUF-2 (forward: 5′-GATGCTGCTCCAGAAGA-3′; reverse: 5′-ACCTTCTGGCAATTCAATC-3′)
Expression Vectors	pQE-30 Xa (for A. baumannii EF-Tu); pBCMAT (for Bifidobacterium promoters)

Canonical and Moonlighting Functions

Translation Elongation:
- Binds GTP and transports aminoacyl-tRNAs to ribosomes .
- Accounts for up to 10% of cytoplasmic protein in some bacteria .
Surface Exposure and Host Interactions:
- EF-Tu in pathogens like Acinetobacter baumannii binds plasminogen and fibronectin, aiding virulence .
- Potential roles in modulating host immune responses or intestinal barrier integrity in B. adolescentis .
Stress Response:
- B. adolescentis strains with tuf operon genes may exhibit enhanced oxidative stress tolerance .

Research Applications and Findings

Recombinant EF-Tu has been leveraged in diverse studies to explore its utility:

Application	Methodology	Outcome
Reporter Systems	tuf promoter cloned into pBCMAT plasmids to drive chloramphenicol acetyltransferase (CAT) expression	High CAT activity in B. adolescentis, confirming tuf promoter strength
Species Identification	PCR-DGGE targeting tuf regions to differentiate Bifidobacterium species	Resolved 13 Bifidobacterium species, including B. adolescentis
Antibiotic Resistance	tuf gene analysis in B. adolescentis genomes to predict resistance markers	Identified rpoB mutations conferring rifampicin resistance

Limitations

Low Adhesion: B. adolescentis strains show weak adhesion to intestinal epithelia, limiting therapeutic efficacy .
Oxidative Stress Sensitivity: Requires thioredoxin reductase (TrxB) for survival in aerobic conditions .

Research Gaps

Structural Characterization: No crystallography or NMR data for B. adolescentis EF-Tu.
Moonlighting Mechanisms: Unexplored roles in immune modulation or barrier repair.
Therapeutic Potential: Lack of in vivo studies on recombinant EF-Tu in IBD models.

Product Specs

Form

Lyophilized powder. We will ship the available format, but please specify any format requirements when ordering.

Lead Time

Delivery times vary by purchase method and location. Consult your local distributor for specifics. Proteins are shipped with blue ice packs by default. Request dry ice in advance (extra fees apply).

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Default glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. Specify your preferred tag type during ordering, and we will prioritize its development.

Synonyms

tuf; BAD_0533Elongation factor Tu; EF-Tu

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-399

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)

Target Names

tuf

Target Protein Sequence

MAKEKYERTK PHVNIGTIGH VDHGKTTLTA AISKVLHEEY PDVNPEYDFN QIDSAPEEAA RGITINIAHI EYQTEKRHYA HVDCPGHADF VKNMITGAAQ MDGAILVVAA TDGPMAQTRE HVLLARQVGV PKILVALNKC DMVDDDELIE LVEEEVRDLL DENGFDRDCP VIHVSAYGAL HDDAPDHEKW VEQIKKLMDA VDDYIPTPVH DLDKPFLMPI EDVFTISGRG TVVTGRVERG KLPVNSNVEI VGIRPTQTTT VTSIETFHKQ MDECEAGDNT GLLLRGINRD QVERGQVLAA PGSVTPHTKF EGEVYVLTKD EGGRHSPFFS NYRPQFYFRT TDVTGVITLP EGVEMVQPGD HATFGVELIQ PIAMEEGLTF AVREGGHTVG SGRVTKIIE

Uniprot No.

A1A0T1

Target Background

Function

This protein facilitates GTP-dependent binding of aminoacyl-tRNA to the ribosomal A-site during protein synthesis.

Database Links

KEGG: bad:BAD_0533

STRING: 367928.BAD_0533

Protein Families

TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-Tu/EF-1A subfamily

Subcellular Location

Cytoplasm.

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Recombinant Bifidobacterium adolescentis Elongation factor Tu (tuf)

Description

Introduction to EF-Tu and Recombinant Production

**Key Features of B. adolescentis EF-Tu**

Genomic Context and Expression Systems

Canonical and Moonlighting Functions

Research Applications and Findings

Limitations

Research Gaps

Product Specs

Target Background

Quick Inquiry

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