Recombinant Bifidobacterium adolescentis Triosephosphate isomerase (tpiA)

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Cat. No.

BT38487

Source

Yeast

Synonyms

tpiA; BAD_0834; Triosephosphate isomerase; TIM; TPI; EC 5.3.1.1; Triose-phosphate isomerase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT38487

Source

E.coli

Synonyms

tpiA; BAD_0834; Triosephosphate isomerase; TIM; TPI; EC 5.3.1.1; Triose-phosphate isomerase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT38487

Source

E.coli

Synonyms

tpiA; BAD_0834; Triosephosphate isomerase; TIM; TPI; EC 5.3.1.1; Triose-phosphate isomerase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT38487

Source

Baculovirus

Synonyms

tpiA; BAD_0834; Triosephosphate isomerase; TIM; TPI; EC 5.3.1.1; Triose-phosphate isomerase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT38487

Source

Mammalian cell

Synonyms

tpiA; BAD_0834; Triosephosphate isomerase; TIM; TPI; EC 5.3.1.1; Triose-phosphate isomerase

Purity

>85% (SDS-PAGE)

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Description

Enzymatic Role and Biological Significance

Triosephosphate isomerase (TIM) is essential for energy production, ensuring metabolic flux through glycolysis and gluconeogenesis. In B. adolescentis, tpiA supports carbohydrate metabolism, enabling the bacterium to thrive in the competitive gut environment by optimizing energy extraction from dietary polysaccharides . Recombinant expression of tpiA facilitates detailed biochemical studies and potential biotechnological applications, such as metabolic engineering of probiotics .

Biochemical Properties

Recombinant tpiA can be expressed in Escherichia coli systems, purified via affinity chromatography, and characterized kinetically:

Catalytic Efficiency: For BmTIM, $K_m = 0.47 \, \text{mM}$ and $V_{\text{max}} = 6031 \, \mu\text{mol min}^{-1} \, \text{mg}^{-1}$ with glyceraldehyde-3-phosphate . Similar values are expected for B. adolescentis tpiA.
pH and Temperature Optima: TIMs generally function optimally near physiological pH (6.5–7.5) and temperatures (30–50°C) .

Table 2: Kinetic Parameters of TIM Enzymes

Enzyme Source	Substrate	$K_m \, (\text{mM})$	$V_{\text{max}} \, (\mu\text{mol min}^{-1} \, \text{mg}^{-1})$
B. adolescentis tpiA	Glyceraldehyde-3P	~0.5 (Predicted)	~6000 (Predicted)
R. microplus TIM	Glyceraldehyde-3P	0.47	6031
Human TIM	Glyceraldehyde-3P	0.18	8400

Functional and Applied Research

While direct studies on B. adolescentis tpiA are sparse, insights can be extrapolated from related work:

Metabolic Engineering: Recombinant TIMs are critical in synthetic pathways for biofuel production (e.g., n-butanol in cyanobacteria) .
Probiotic Enhancement: B. adolescentis strains engineered with recombinant enzymes could improve metabolic versatility or therapeutic efficacy in gut microbiota modulation .
Prebiotic Metabolism: B. adolescentis β-glucosidases and xylosidases have been recombinantly studied for oligosaccharide hydrolysis , suggesting tpiA could similarly aid in prebiotic utilization.

Research Gaps and Future Directions

Structural Resolution: Crystallographic studies of B. adolescentis tpiA are needed to confirm its dimeric architecture and active-site residues.
Substrate Specificity: Comparative assays with homologs could reveal unique kinetic adaptations to the gut environment.
In Vivo Applications: Linking tpiA activity to B. adolescentis colonization efficiency or host metabolic benefits remains unexplored .

Product Specs

Form

Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering.

Lead Time

Delivery time varies based on purchasing method and location. Consult local distributors for specific delivery times. Proteins are shipped with blue ice packs by default. Request dry ice in advance for an extra fee.

Notes

Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer ingredients, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

tpiA; BAD_0834; Triosephosphate isomerase; TIM; TPI; EC 5.3.1.1; Triose-phosphate isomerase

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-264

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)

Target Names

tpiA

Target Protein Sequence

MGPKRIPLVA GNWKMNFDHL EATYFVQQLA WNLRAIHFDY KRCEIALMPS FTSLRSVQVA VESDNLKIRY GAQAVSVTSQ GAFTGDVSAD MIAHLGCSYV IVGHSERRKY HPEDDANIVD QVRAVLAAGM QPILCVGESY EERRKGIELD FAVGQVHDVT RDLSDEEAAK LIVAYEPVWA IGTGMVATPQ SAQDAARAIR NDLSDTFGTR VGETVRILYG GSVSSKNAVE LINEPDVDGF LIGGSALKVD ELTRICKLTL ETTA

Uniprot No.

A1A1N2

Target Background

Function

Involved in gluconeogenesis. Catalyzes the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).

Database Links

KEGG: bad:BAD_0834

STRING: 367928.BAD_0834

Protein Families

Triosephosphate isomerase family

Subcellular Location

Cytoplasm.

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Recombinant Bifidobacterium adolescentis Triosephosphate isomerase (tpiA)

Description

Enzymatic Role and Biological Significance

Biochemical Properties

Table 2: Kinetic Parameters of TIM Enzymes

Functional and Applied Research

Research Gaps and Future Directions

Product Specs

Target Background

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