Recombinant Bifidobacterium longum subsp. infantis Prolipoprotein diacylglyceryl transferase (lgt)
Description
Definition and Functional Role
Recombinant Bifidobacterium longum subsp. infantis Prolipoprotein diacylglyceryl transferase (lgt) is a bacterial enzyme responsible for lipidating prolipoproteins, a critical step in the maturation of bacterial membrane proteins. This post-translational modification involves transferring a diacylglyceryl group to the sulfhydryl group of a conserved cysteine residue in prolipoproteins, anchoring them to the cell membrane . The enzyme is encoded by the lgt gene (Blon_1369 in strain ATCC 15697) and plays a role in cell envelope biogenesis, virulence, and host-microbe interactions .
Biological Context in B. infantis
B. longum subsp. infantis ATCC 15697 is a keystone gut symbiont in breastfed infants, specializing in human milk oligosaccharide (HMO) metabolism . While the lgt enzyme itself is not directly linked to HMO utilization, its role in membrane protein maturation supports:
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Oligosaccharide Transport: Membrane-bound solute-binding proteins (SBPs) critical for HMO uptake require proper lipidation for functionality .
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Host Interaction: Lipoproteins contribute to bacterial adhesion and immune modulation, which are essential for colonization .
Current Uses
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Research Tool: Recombinant lgt is used to study lipoprotein processing in Bifidobacterium and other Gram-positive bacteria .
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Structure-Function Studies: Investigating how lipid modification affects membrane protein localization and stability .
Key Research Findings
Research Gaps and Future Directions
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Mechanistic Studies: Limited data exist on substrate specificity or regulatory interactions in B. infantis.
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Therapeutic Potential: No direct evidence links lgt to probiotic efficacy, though strain-specific genomic clusters (e.g., HMO utilization) suggest synergies .
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Structural Biology: High-resolution crystal structures of lgt remain unpublished.
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them when placing your order. We will prepare the product according to your specifications.
Note: All our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance. Additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: bln:Blon_1369
