Recombinant Bombyx mori 5-hydroxytryptamine receptor
Description
Functional and Pharmacological Profile
Research on related serotonin receptors in Bombyx mori highlights their role in modulating physiological processes:
Immune Regulation
Serotonin (5-HT) in insects regulates hemocyte phagocytosis, a key immune response mechanism. Studies in Drosophila melanogaster and Pieris rapae demonstrate that 5-HT signaling via 5-HT1B and 5-HT2B receptors enhances phagocytosis during bacterial infections . While the recombinant protein’s specific subtype is not explicitly defined in product specifications, its structural homology to these receptors suggests potential utility in studying immune-related signaling.
Locomotion and Behavior
In Bombyx mori, the 5-HT1A receptor subtype (a homolog of the recombinant protein) is linked to locomotor activity. Pharmacological studies reveal:
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Agonists: Pimozide > 8-OH-DPAT > 5-MeOT ~ α-m-5-HT (EC₅₀ = 117 nM for 5-HT) .
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Antagonists: WAY-100635 > prazosin > SB-269970 > methiothepin .
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Functional Impact: Antagonist injection reduces larval motility and adult courtship activity, while RNAi-mediated knockdown of Bm5-HT1A lowers locomotion .
Signaling Mechanisms
The recombinant protein’s activity is characterized by its inhibition of forskolin-stimulated cAMP synthesis, a hallmark of Gαi/o-coupled 5-HT receptors. This aligns with the 5-HT1A receptor’s role in reducing intracellular cAMP levels in HEK 293 cell models .
Research Applications
The recombinant protein serves as a versatile tool in molecular biology and pharmacology:
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them in your order. We will fulfill your request to the best of our ability.
Note: All our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance. Additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. For lyophilized form, the shelf life is 12 months at -20°C/-80°C.
