Recombinant Bordetella avium Glycerol-3-phosphate acyltransferase (plsY)
Description
Enzymatic Function and Biological Role
PlsY belongs to the family of acyltransferases (EC 2.3.1.n3) and operates in the Bordetella avium glycerolipid synthesis pathway . Key features include:
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Substrate specificity: Utilizes acyl-phosphate as the acyl donor (not acyl-CoA or acyl-ACP) .
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Catalytic activity: Converts acyl-phosphate + G3P → LPA + inorganic phosphate .
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Membrane topology: Contains five transmembrane segments, with conserved cytoplasmic motifs critical for substrate binding and catalysis .
Critical residues:
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Motif 1: Essential serine (Ser) and arginine (Arg) residues for catalysis .
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Motif 2: Phosphate-binding loop with glycine residues critical for G3P binding .
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Motif 3: Histidine (His) and asparagine (Asn) for structural integrity .
Recombinant Protein Characteristics
Recombinant PlsY from B. avium (strain 197N) is produced in E. coli systems for research applications. Key properties include:
Research Applications
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ELISA and immunodetection: Recombinant PlsY is used as an antigen in immunological assays to study antibody responses .
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Enzyme kinetics: Mutagenesis studies (e.g., glycine-to-alanine substitutions in Motif 2) reveal defects in Km for G3P binding .
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Inhibition studies: Noncompetitive inhibition by palmitoyl-CoA highlights regulatory mechanisms .
Biotechnological Relevance
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Vaccine development: While not directly used in vaccines, B. avium recombinant proteins (e.g., ompA-Fc fusions) leverage similar expression systems (Pichia pastoris) to enhance immunogenicity .
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Antimicrobial research: Understanding PlsY’s role in membrane biogenesis aids in targeting bacterial lipid metabolism for drug discovery .
Comparative Analysis
PlsY differs from eukaryotic GPATs, which typically acylate the sn-1 position of G3P. In contrast, some plant GPATs (e.g., GPAT4/6) exhibit sn-2 preference and phosphatase activity .
Future Directions
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it when placing the order. We will prepare according to your request.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance. Additional fees will apply.
The tag type is determined during the production process. If you have a specific tag type in mind, please inform us. We will prioritize developing the specified tag if possible.
Target Background
KEGG: bav:BAV1982
STRING: 360910.BAV1982
