Recombinant Bordetella pertussis tRNA-specific 2-thiouridylase mnmA (mnmA)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless dry ice is requested in advance. Additional fees apply for dry ice shipping.
Note: All proteins are shipped with standard blue ice packs unless dry ice is requested in advance. Additional fees apply for dry ice shipping.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, which can be used as a guideline.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer components, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
mnmA; BP2893tRNA-specific 2-thiouridylase MnmA; EC 2.8.1.13
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-371
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Target Names
mnmA
Target Protein Sequence
MPSNPTRKGR VVVGMSGGVD SSVTAWLLKQ QGYEVVGLFM KNWEDDDDSE YCSTRQDLLD
AASVADLVGV EFEYVNFAAE YKDRVFAEFL REYSAGRTPN PDVLCNAEIK FKAFLDHAMA
LGAEHIATGH YARVRTVETP AGPRHQLLKA LDDTKDQSYF LHRLNQVQLA RTLFPLGELR
KTEVRRIAHE IGLHNAAKKD STGICFIGER PFREFLNRYL PSEPGPILTP EGQRVGTHHG
LSFYTLGQRK GLGVGGVKGR QRDDGTAEAW YAARKDLARN VLYVVQGHDH PWLLSAQLQA
QDASWIAGEP PAAGAYGAKT RYRQADAACR LDQAGGERFA LAFEQAQWAV TPGQSAVLYD
GEVCLGGGII I
Uniprot No.
Target Background
Function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, resulting in the formation of s²(2)U34.
Database Links
KEGG: bpe:BP2893
STRING: 257313.BP2893
Protein Families
MnmA/TRMU family
Subcellular Location
Cytoplasm.
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