Recombinant Bovine Endothelin B receptor-like protein 2 (GPR37L1)
Description
Production Methods
Recombinant bovine GPR37L1 is engineered for high yield and specificity:
Functional Roles
GPR37L1 is implicated in diverse physiological processes:
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Neurological Regulation: Modulates cerebellar development, Bergmann glia maturation, and glutamate homeostasis .
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Cardiovascular Control: Regulates blood pressure homeostasis via central mechanisms .
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Neuroprotection: Binds prosaposin/prosaptide to reduce oxidative stress in astrocytes .
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Epilepsy and Migraine: Rare variants correlate with progressive myoclonus epilepsy and migraine .
Signaling Pathways and Ligand Interactions
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Prosaposin/Prosaptide Binding: Activates Gαi-mediated pathways, inhibiting cAMP (EC₅₀ ~10 nM) and phosphorylating ERK1/2 .
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Syntenin-1 Interaction: Enhances plasma membrane trafficking by 10-fold in heterologous systems .
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Ion Transport: Regulates renal sodium reabsorption, linking to hypertension mechanisms .
Functional Assays
Potential Applications
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Diagnostic Tools: Commercial ELISA kits enable GPR37L1 quantification in serum, plasma, and tissues .
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Therapeutic Targets:
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Drug Discovery: High-purity recombinant protein supports ligand screening and structural studies .
Challenges and Future Directions
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Expression Limitations: Poor native trafficking necessitates truncation or co-expression with syntenin-1 for functional studies .
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Ligand Specificity: Despite prosaposin pairing, endogenous ligands in peripheral tissues remain unconfirmed .
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Species-Specific Variations: Functional differences between bovine and human isoforms require further characterization .
Future research should prioritize crystallography to resolve bovine GPR37L1’s active-state structure and in vivo validation of its neuroprotective effects .
Product Specs
Please note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them in your order notes, and we will fulfill them accordingly.
Note: All of our proteins are shipped with standard blue ice packs by default. If dry ice shipping is required, please inform us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C, while the shelf life of lyophilized form is 12 months at -20°C/-80°C.
