Recombinant Bovine PRA1 family protein 2 (PRAF2)
Description
Protein Transport Regulation
PRAF2 acts as a "gatekeeper" for chemokine receptors like CCR5, retaining them in the ER via transmembrane domain interactions . This contrasts with CD4, which promotes CCR5 plasma membrane export . In vesicular traffic, PRAF2 interacts with Rab GTPases and modulates ER/Golgi trafficking pathways .
Apoptosis Modulation
PRAF2 binds anti-apoptotic proteins Bcl-xL and Bcl-2 through their transmembrane domains, counteracting their survival signals . Overexpression induces apoptosis by triggering Bax mitochondrial translocation and caspase activation, which is inhibited by wild-type Bcl-xL but not its transmembrane domain-deleted mutant .
Viral Interactions
PRAF2 binds HPV E5 oncoprotein, with amino acids 135–160 critical for interaction . This interaction may disrupt viral lifecycle processes, such as E1^E4 expression in organotypic rafts .
Research Applications
Recombinant bovine PRAF2 is utilized in:
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Cell-Surface Protein Localization Studies: BRET assays to monitor CCR5 retention in the ER .
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Apoptosis Pathway Analysis: Co-immunoprecipitation (Co-IP) and PARP cleavage assays to assess pro-apoptotic activity .
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Viral Oncology Research: Proximity-dependent biotinylation (BioID) to map interactomes with HPV E5 .
Cancer and Therapeutic Relevance
PRAF2 overexpression is linked to poor prognosis in hepatocellular carcinoma, glioblastoma, and neuroblastoma . In cervical cancer models, its knockdown reduces colony formation and migration, suggesting oncogenic potential . Conversely, its pro-apoptotic activity in normal cells highlights therapeutic potential for targeting apoptosis-resistant cancers .
Experimental Considerations
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Expression Systems: Recombinant PRAF2 is produced in S2 insect cells or mammalian systems (e.g., HEK293) .
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Antibodies: Polyclonal rabbit antibodies (e.g., Sigma-Aldrich ABC95) target native and recombinant PRAF2 for Western blot and immunohistochemistry .
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Species Cross-Reactivity: Bovine PRAF2 antibodies show predicted reactivity with human, mouse, and rat proteins .
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have a specific format preference, please indicate it in your order remarks. We will fulfill your request if possible.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please communicate with us in advance as additional fees will apply.
Generally, liquid forms have a shelf life of 6 months at -20°C/-80°C. Lyophilized forms have a shelf life of 12 months at -20°C/-80°C.
