Recombinant Bovine V-type proton ATPase 16 kDa proteolipid subunit (ATP6V0C)
Description
Role in V-ATPase
ATP6V0C is a core component of the V₀ domain of V-ATPase, a multi-subunit enzyme responsible for acidifying intracellular organelles (e.g., lysosomes, Golgi apparatus) by transporting protons across membranes . The V₀ domain consists of five subunits (a, b, c, c', d), where ATP6V0C forms the proteolipid c-ring, essential for proton translocation .
Expression Platforms
Recombinant bovine ATP6V0C is produced using diverse host systems:
Cloning and Vector Design
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Vectors: pCMV6-AC-GFP or similar plasmids with neomycin resistance for mammalian selection .
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Tagging: C-terminal GFP or Myc-DDK tags for purification and localization studies .
Cancer Metabolism
ATP6V0C interacts with pyruvate kinase M2 (PKM2), enhancing aerobic glycolysis in esophageal cancer cells. Silencing ATP6V0C reduces lactate production, extracellular acidification, and PKM2 phosphorylation (Tyr105), impairing tumor growth .
Neurological Disorders
Mutations in ATP6V0C (e.g., Ala22Thr) disrupt proton transport, leading to epilepsy and neurodevelopmental abnormalities. Functional studies in Drosophila and C. elegans show increased seizure-like behavior and reduced lifespan .
Autophagy Regulation
In neuroblastoma cells, ATP6V0C knockdown blocks lysosomal acidification, inhibiting autophagic flux and increasing α-synuclein and APP C-terminal fragment accumulation .
Comparative Analysis: Bovine vs. Human ATP6V0C
Challenges and Future Directions
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Stability: Recombinant ATP6V0C requires careful handling to maintain membrane-integrated conformation .
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Functional Validation: Activity assays (e.g., LysoSensor fluorescence, V-ATPase inhibitor response) are critical for confirming functionality .
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Therapeutic Potential: Targeting ATP6V0C in cancer or neurodegenerative diseases may leverage its role in pH regulation and metabolism .
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them when placing your order, and we will accommodate your request.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance, as additional fees may apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
