Recombinant Bradyrhizobium japonicum PKHD-type hydroxylase blr3905 (blr3905)
Description
Introduction
Bradyrhizobium japonicum is a bacterium notable for its symbiotic relationship with soybean plants, where it facilitates nitrogen fixation in root nodules . The blr3905 gene in B. japonicum encodes a PKHD-type hydroxylase, an enzyme that may be involved in cellular responses to DNA damage . PKHD-type hydroxylases, such as YbiX in Escherichia coli, are 2-oxoglutarate-dependent dioxygenases and possess iron ion-binding capabilities .
Identification and Basic Properties
The PKHD-type hydroxylase YbiX, which shares functional similarities with blr3905, has the following characteristics :
| Identification | Details |
|---|---|
| Name | PKHD-type hydroxylase YbiX |
| Synonyms | Not Available |
| Gene Name | ybiX |
| Enzyme Class | Not Available |
| General Function | Cellular response to DNA damage stimulus |
| Specific Function | Not Available |
| Cellular Location | Not Available |
| Protein Residues | 225 |
| Protein Molecular Weight | 25528 Da |
Functional Roles and Significance
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Nitrogen Fixation: B. japonicum's primary role is to establish a symbiotic relationship with soybean plants, enabling the conversion of atmospheric nitrogen into ammonia, a form usable by the plant .
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Stress Response: Trehalose accumulation, influenced by genes like otsA, treS, and treY in B. japonicum, enhances survival under salinity and desiccation stresses, thereby supporting the bacterium's symbiotic proficiency .
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Proline Biosynthesis: The proC gene in B. japonicum is essential for symbiosis with soybeans, suggesting that the bacterium cannot obtain enough proline from the host plant to satisfy its auxotrophy if this gene is mutated .
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Nodulation and Competition: Modified strains of B. japonicum can enhance nitrogen fixation and compete for nodulation . For example, strain Bj 5019 can outcompete indigenous Bradyrhizobia for nodulation of soybean plants .
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Induction of nod Genes: Flavonoids like daidzein and genistein induce common nod genes in B. japonicum, which are crucial for the early stages of legume nodulation .
Impact of Nutrient Availability
The symbiotic performance of B. japonicum is affected by nutrient availability, with nodulation inhibited by higher nitrogen supply combined with either high or low phosphorus concentrations . Magnesium promotes plant growth and development in combination with high nitrogen in the growth medium .
Product Specs
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