Recombinant Branchiostoma lanceolatum NADH-ubiquinone oxidoreductase chain 6 (ND6)

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Cat. No.

BT2140163

Source

in vitro E.coli expression system

Synonyms

ND6; NAD6; NADH6; NADH-ubiquinone oxidoreductase chain 6; NADH dehydrogenase subunit 6

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Description

Functional Role in Complex I

ND6 is one of seven mitochondrial-encoded subunits in Complex I, which catalyzes NADH oxidation and proton translocation across the inner mitochondrial membrane .

Mechanistic Contributions

Electron Transfer: Participates in the peripheral arm of Complex I, facilitating electron flow from FMN to ubiquinone .
Proton Pumping: Contributes to the transmembrane domain, enabling proton extrusion .

Comparison with Human ND6

Feature	B. lanceolatum ND6	Human ND6 (MT-ND6)
Length	167 aa	172 aa
Gene Location	Nuclear genome (recombinant)	Mitochondrial L-strand (14,149–14,673 bp)
Clinical Relevance	Research tool	Associated with LHON, Leigh syndrome, dystonia
Protein Features	Hydrophobic transmembrane domains	Includes redox-active Fe-S clusters

Applications in Research

SDS-PAGE and ELISA

SDS-PAGE: Used to verify protein purity and molecular weight .
ELISA Kits: Available for quantification (e.g., CSB-EP015082BVZ1 at $1,511.00 for 50 µg) .

Functional Studies

Complex I Assembly: Investigates interactions with nuclear-encoded subunits (e.g., NDUFS3, YME1L1) .
Evolutionary Insights: Compares B. lanceolatum ND6 with human homologs to study mitochondrial evolution .

Handling and Stability

Avoid Freeze-Thaw Cycles: Working aliquots stored at 4°C for ≤1 week .
Buffer Compatibility: Tris/PBS-based buffer with trehalose stabilizes protein structure .

Product Specs

Form

Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.

Lead Time

Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, but this can be adjusted per customer requirements.

Shelf Life

Shelf life depends on several factors, including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.

Storage Condition

Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing.
The specific tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its inclusion.

Synonyms

ND6; NAD6; NADH6; NADH-ubiquinone oxidoreductase chain 6; NADH dehydrogenase subunit 6

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-167

Protein Length

full length protein

Species

Branchiostoma lanceolatum (Common lancelet) (Amphioxus lanceolatum)

Target Names

ND6

Target Protein Sequence

MQMMLMFLLLLAAIMVIRATSPYYGALATAWLALLAALLLLDADIIFPAIILMLIYLGGM LVVFIYSTAYAADLMPLPINLTMSALMASFGVMLITMISSPSIETLCETKPWLVYDMQPS YMLFDIYQRGSSMFIVAVMILTALLFSILEVVSHRQTTMKWFIHSTY

Uniprot No.

P69233

Target Background

Function

Recombinant Branchiostoma lanceolatum NADH-ubiquinone oxidoreductase chain 6 (ND6): A core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). It's considered part of the minimal assembly necessary for catalytic activity. Complex I facilitates electron transfer from NADH to the respiratory chain, with ubiquinone believed to be the immediate electron acceptor.

Protein Families

Complex I subunit 6 family

Subcellular Location

Mitochondrion membrane; Multi-pass membrane protein.

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Recombinant Branchiostoma lanceolatum NADH-ubiquinone oxidoreductase chain 6 (ND6)

Description

Functional Role in Complex I

Mechanistic Contributions

Comparison with Human ND6

Applications in Research

SDS-PAGE and ELISA

Functional Studies

Handling and Stability

Product Specs

Target Background

Quick Inquiry

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