Recombinant Brucella abortus biovar 1 Glycine dehydrogenase [decarboxylating] (gcvP), partial

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Cat. No.
BT44118
Source
Yeast
Synonyms
gcvP; BruAb2_0506Glycine dehydrogenase; decarboxylating; EC 1.4.4.2; Glycine cleavage system P-protein; Glycine decarboxylase; Glycine dehydrogenase; aminomethyl-transferring)
Purity
>85% (SDS-PAGE)
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Cat. No.
BT44118
Source
E.coli
Synonyms
gcvP; BruAb2_0506Glycine dehydrogenase; decarboxylating; EC 1.4.4.2; Glycine cleavage system P-protein; Glycine decarboxylase; Glycine dehydrogenase; aminomethyl-transferring)
Purity
>85% (SDS-PAGE)
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Cat. No.
BT44118
Source
E.coli
Synonyms
gcvP; BruAb2_0506Glycine dehydrogenase; decarboxylating; EC 1.4.4.2; Glycine cleavage system P-protein; Glycine decarboxylase; Glycine dehydrogenase; aminomethyl-transferring)
Purity
>85% (SDS-PAGE)
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Cat. No.
BT44118
Source
Baculovirus
Synonyms
gcvP; BruAb2_0506Glycine dehydrogenase; decarboxylating; EC 1.4.4.2; Glycine cleavage system P-protein; Glycine decarboxylase; Glycine dehydrogenase; aminomethyl-transferring)
Purity
>85% (SDS-PAGE)
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Cat. No.
BT44118
Source
Mammalian cell
Synonyms
gcvP; BruAb2_0506Glycine dehydrogenase; decarboxylating; EC 1.4.4.2; Glycine cleavage system P-protein; Glycine decarboxylase; Glycine dehydrogenase; aminomethyl-transferring)
Purity
>85% (SDS-PAGE)
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Description

Biochemical Characteristics of gcvP

gcvP is the P-protein of the GCS, which binds glycine via its pyridoxal phosphate (PLP) cofactor . Key features include:

  • Function: Converts glycine into 5,10-methylenetetrahydrofolate, supporting nucleotide and amino acid biosynthesis .

  • Structure: Contains a PLP-binding domain critical for decarboxylation activity .

  • Recombinant Form: Partial sequences are often engineered for functional studies, omitting non-essential regions while retaining catalytic domains .

Role in Brucella abortus Pathogenesis

gcvP is essential for chronic persistence in hosts:

  • Mouse Infection Models:

    • Mutants lacking gcvB (a homolog of gcvP) established initial infection but failed to persist in spleens at 8 weeks post-infection .

    • This defect mirrors Mycobacterium tuberculosis GCS mutants, suggesting conserved persistence mechanisms among intracellular pathogens .

Table 1: Impact of gcvP Disruption on B. abortus Virulence

ParameterWild-Type B. abortusΔgcvP Mutant
Spleen colonization (2 weeks)4.75 ± 0.12 log CFU4.70 ± 0.25 log CFU
Spleen colonization (8 weeks)4.22 ± 0.58 log CFUNot detected
Mouse survival rate100% (12 weeks)Cleared by week 10
Data derived from murine infection studies .

Vaccine Development

Recombinant GCS proteins like gcvP are explored as subunit vaccine candidates:

  • Immunogenicity: Recombinant B. abortus proteins (e.g., r-glk) induce Th1 immune responses and protect against challenge .

  • Cross-Protection: GCS enzymes are conserved across Brucella species, making them broad-spectrum antigen targets .

Host-Pathogen Interactions

  • Apoptosis Modulation: GCS homologs in Mycoplasma inhibit host apoptosis by stabilizing endoplasmic reticulum kinases . While unconfirmed for Brucella gcvP, similar mechanisms may enhance intracellular survival.

  • Metabolic Adaptations: gcvP supports gluconeogenesis in nutrient-limited intracellular niches, as shown by proteomic studies of B. abortus-infected macrophages .

Technical Considerations in Recombinant Production

  • Expression Systems: E. coli BL21 is commonly used for recombinant Brucella protein expression .

  • Enzymatic Assays: GFP-refolding and CsA-inhibition tests validate PPIase-like activity in related proteins (e.g., CypB) .

Knowledge Gaps and Future Directions

  • Structural Data: Full-length gcvP structure remains unresolved, limiting drug design efforts .

  • In Vivo Validation: Recombinant gcvP’s efficacy as a vaccine antigen or therapeutic target requires further animal trials .

Product Specs

Form
Lyophilized powder. We will ship the available format, but please specify any format requirements when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult local distributors for specific times. Proteins ship with blue ice packs; request dry ice in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. Specify your preferred tag type, and we will prioritize its development.
Synonyms
gcvP; BruAb2_0506Glycine dehydrogenase; decarboxylating; EC 1.4.4.2; Glycine cleavage system P-protein; Glycine decarboxylase; Glycine dehydrogenase; aminomethyl-transferring)
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Brucella abortus biovar 1 (strain 9-941)
Target Names
gcvP
Uniprot No.
P62920

Target Background

Function
The glycine cleavage system degrades glycine. The P protein binds glycine's alpha-amino group via its pyridoxal phosphate cofactor, releasing CO2. The remaining methylamine is transferred to the H protein's lipoamide cofactor.
Database Links

KEGG: bmb:BruAb2_0506

Protein Families
GcvP family

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