Recombinant Brucella abortus biovar 1 Probable ABC transporter permease protein BruAb2_1124 (BruAb2_1124)
Description
Functional Role in Brucella Biology
BruAb2_1124 is annotated as a probable ABC transporter permease, implicating it in nutrient uptake or efflux of metabolites critical for bacterial survival. Key functional insights include:
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Mechanism: ABC transporters use ATP binding/hydrolysis to alternate between inward- and outward-facing conformations, enabling substrate translocation across membranes .
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Pathogenicity Link: In Brucella, ABC transporters are hypothesized to import essential nutrients (e.g., vitamins, metals) during intracellular infection, facilitating host persistence .
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Biochemical Role: BruAb2_1124 may partner with other subunits (e.g., solute-binding proteins) to form a functional transporter complex, though specific substrates remain unidentified .
Research Applications
This recombinant protein is critical for:
Vaccine Development
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Serves as an antigen candidate for brucellosis vaccine research due to its surface-exposed epitopes in Brucella .
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Used in immunogenicity studies to assess host antibody responses .
Mechanistic Studies
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Facilitates structural and functional analyses of ABC transporter dynamics in Gram-negative bacteria .
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Enables drug discovery efforts targeting bacterial nutrient uptake pathways .
Diagnostic Assays
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Employed in ELISA-based detection systems (e.g., Cat# CSB-CF685026BWS) to identify Brucella-specific antibodies in clinical samples .
Key Research Findings and Gaps
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Homology: BruAb2_1124 shares sequence similarity with ABC permeases in Salmonella and E. coli, suggesting conserved roles in stress adaptation .
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Knowledge Gaps:
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Therapeutic Potential: Targeting BruAb2_1124 could disrupt bacterial nutrient acquisition, but inhibitor design requires structural resolution .
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it during order placement, and we will fulfill your request.
Note: All protein shipments are standardly packaged with blue ice packs. If you require dry ice shipping, please inform us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: bmb:BruAb2_1124
