Recombinant Brucella suis biovar 1 Elongation factor Ts (tsf)

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In Stock
Cat. No.
BT46619
Source
Yeast
Synonyms
tsf; BR1161; BS1330_I1157Elongation factor Ts; EF-Ts
Purity
>85% (SDS-PAGE)
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Cat. No.
BT46619
Source
E.coli
Synonyms
tsf; BR1161; BS1330_I1157Elongation factor Ts; EF-Ts
Purity
>85% (SDS-PAGE)
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Cat. No.
BT46619
Source
E.coli
Synonyms
tsf; BR1161; BS1330_I1157Elongation factor Ts; EF-Ts
Purity
>85% (SDS-PAGE)
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Cat. No.
BT46619
Source
Baculovirus
Synonyms
tsf; BR1161; BS1330_I1157Elongation factor Ts; EF-Ts
Purity
>85% (SDS-PAGE)
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Cat. No.
BT46619
Source
Mammalian cell
Synonyms
tsf; BR1161; BS1330_I1157Elongation factor Ts; EF-Ts
Purity
>85% (SDS-PAGE)
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Description

Definition and Biological Role

Elongation Factor Ts (EF-Ts), encoded by the tsf gene, is a critical component of bacterial protein synthesis. It functions as a guanine nucleotide exchange factor (GEF) for elongation factor Tu (EF-Tu), catalyzing the replacement of GDP with GTP on EF-Tu to regenerate the active EF-Tu·GTP complex. This process is essential for the cyclical recruitment of aminoacyl-tRNA (aa-tRNA) to the ribosome during translation .

In Brucella suis biovar 1, EF-Ts plays a conserved role in maintaining translational fidelity and efficiency. The tsf gene is part of the core genome of Brucella spp., with high sequence homology to EF-Ts in other bacteria .

Recombinant Production and Applications

Recombinant EF-Ts from B. suis biovar 1 is engineered for studies on bacterial translation mechanisms, antibiotic resistance, and vaccine development.

Key Production Steps:

  1. Gene Cloning: The tsf gene is amplified from B. suis biovar 1 genomic DNA .

  2. Expression: Typically expressed in E. coli systems using plasmids with inducible promoters (e.g., T7 or arabinose).

  3. Purification: Affinity chromatography (e.g., His-tag systems) yields >85% purity.

Biochemical Properties:

  • Molecular Weight: ~35 kDa (predicted) .

  • Stability: Requires GTP/Mg²⁺ for functional conformation .

  • Activity: Accelerates GDP-GTP exchange on EF-Tu by 100–1,000-fold .

Role in Pathogenesis

EF-Ts is critical for B. suis virulence, as it ensures efficient protein synthesis under host stress conditions. Knockout mutants show reduced survival in macrophages .

Antibiotic Target Potential

EF-Ts is a candidate for novel antibiotics due to its essential role in translation. Inhibitors targeting EF-Ts/EF-Tu interactions could disrupt bacterial growth .

CompoundEffect on EF-TsReference
KirromycinStabilizes EF-Tu·GDP, blocking EF-Ts action
GE2270ABinds EF-Tu, indirectly affecting EF-Ts

Vaccine Development

Recombinant EF-Ts is being explored as a subunit vaccine antigen. Preliminary studies in mice show it elicits Th1 immune responses, crucial for intracellular pathogen clearance .

Challenges and Future Directions

  • Structural Studies: No crystal structure of B. suis EF-Ts is available; homology modeling relies on E. coli data .

  • Diagnostic Utility: Cross-reactivity with EF-Ts from other Brucella species complicates serodiagnostics .

  • Therapeutic Optimization: Improving inhibitor specificity to avoid off-target effects in eukaryotic cells .

Product Specs

Form
Lyophilized powder. We will ship the available format, but please note any format requirements when ordering, and we will accommodate your request.
Lead Time
Delivery times vary by purchase method and location. Contact your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. Request dry ice in advance for an extra fee.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during manufacturing. If you require a specific tag, please let us know, and we will prioritize its development.
Synonyms
tsf; BR1161; BS1330_I1157Elongation factor Ts; EF-Ts
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-305
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Brucella suis biovar 1 (strain 1330)
Target Names
tsf
Target Protein Sequence
MSISASLVKE LRDLTGAGMM DCKAALAATE GKIEAAVDWL RAKGIAKADK KAGRTAAEGL VGVAASGNKA VVVEVNSETD FVARNDAFQE LVRKIAQAAL STDGSSEAVA NANVDGKTVT EAAKDAVATI GENISFRRSA ALSVPQGVVA TYIHNGVADG LGKLGVLVAI ETAGDAEAAQ AFGRQVAMHV AAVNPLALTS ADVNPEAAER EKAIFIDQAR QSGKPDNIIE KMVEGRMRKF YEEVVLLSQA FVINPDLTVE AALKDAEKAI GAPAKITGFA RIALGEGIEK EESDFAAEVA AAAKG
Uniprot No.
P64049

Target Background

Function
Associates with the EF-Tu.GDP complex and facilitates GDP to GTP exchange. Remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex until GTP hydrolysis on the ribosome.
Database Links

KEGG: bms:BR1161

Protein Families
EF-Ts family
Subcellular Location
Cytoplasm.

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