Recombinant Brucella suis biovar 1 UPF0283 membrane protein BR1033/BS1330_I1029 (BR1033, BS1330_I1029)
Description
General Information
Recombinant Brucella suis biovar 1 UPF0283 membrane protein BR1033/BS1330_I1029, also referred to as BR1033 or BS1330_I1029, is a protein expressed in Brucella suis biovar 1 . It is sometimes produced using recombinant DNA technology and tagged with histidine (His) to facilitate purification .
Function and Characteristics
BR1033/BS1330_I1029 is annotated as a UPF0283 membrane protein, indicating it is a protein of unknown function (UPF) with a transmembrane domain . Proteins in this category often have poorly characterized functions but are conserved across species, suggesting they may play essential roles.
Expression and Purification
Recombinant BR1033/BS1330_I1029 is typically produced in E. coli and fused to an N-terminal His tag . The His tag allows for purification using affinity chromatography, where the protein binds to a nickel-charged resin and is later eluted.
Purification Steps:
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E. coli cells expressing the His-tagged BR1033/BS1330_I1029 are lysed.
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The lysate is passed through a nickel affinity column.
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The column is washed to remove unbound proteins.
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BR1033/BS1330_I1029 is eluted using a buffer containing imidazole, which competes with the protein for binding to the nickel ions.
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The eluted protein is dialyzed or buffer-exchanged to remove imidazole and stabilize the protein.
Role in Brucella suis Virulence
Brucella species are facultative intracellular bacteria that cause brucellosis, a zoonotic disease . A key aspect of Brucella virulence is their ability to survive and multiply within host phagocytes . Mutagenesis studies have identified several factors required for virulence, including the type IV secretion system (T4SS) encoded by the virB operon .
While BR1033/BS1330_I1029 is annotated as a UPF0283 membrane protein, other Brucella virulence factors have been identified, such as BvfA (Brucella virulence factor A), a small periplasmic protein essential for the virulence of Brucella suis . A BvfA knockout mutant was highly attenuated in both in vitro macrophage infection assays and in vivo in the murine model of brucellosis .
Potential Applications
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested. Advance notification is required for dry ice shipments, and additional fees will apply.
The tag type will be determined during the production process. If you require a specific tag type, please inform us; we will prioritize developing your specified tag.
Target Background
KEGG: bms:BR1033
