Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum UPF0259 membrane protein BUAP5A_271 (BUAP5A_271)
Description
Origin and Biological Context
BUAP5A_271 is encoded by the BUAP5A_271 gene in Buchnera aphidicola (strain 5A), a bacterium that resides intracellularly in aphids like Acyrthosiphon pisum . Buchnera has undergone extensive genome reduction due to its obligate symbiosis, retaining genes critical for amino acid biosynthesis (e.g., tryptophan) while losing others unnecessary in aphid-host environments . The BUAP5A_271 gene is annotated as a multi-pass membrane protein, though its exact functional role remains unclear .
Recombinant Expression
BUAP5A_271 is heterologously expressed in E. coli using standard recombinant protein protocols . Key production parameters include:
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Tag Information: N-terminal or C-terminal tags (e.g., His-tag) are applied to facilitate purification .
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Yield: Typically supplied in quantities of 50 µg, with custom amounts available .
Immunological Studies
BUAP5A_271 is utilized in ELISA assays to detect specific antibodies or study protein interactions. For example, recombinant proteins like this are critical for developing diagnostics targeting Buchnera-aphid symbiosis .
Symbiosis Research
BUAP5A_271 serves as a model for studying gene loss and functional adaptation in symbiotic bacteria. Buchnera’s reduced genome highlights the trade-offs between retained essential functions and lost non-essential genes .
Functional Gaps and Future Directions
Despite its availability, BUAP5A_271’s precise biological function remains undefined. Key areas for further research include:
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please specify them in your order notes. We will accommodate your request whenever possible.
Note: All our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance, as additional fees may apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: bap:BUAP5A_271
