Recombinant Burkholderia cenocepacia ATP synthase subunit delta (atpH)

Shipped with Ice Packs
In Stock
Cat. No.
BT45819
Source
Yeast
Synonyms
atpH; Bcenmc03_0119ATP synthase subunit delta; ATP synthase F(1) sector subunit delta; F-type ATPase subunit delta; F-ATPase subunit delta
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT45819
Source
E.coli
Synonyms
atpH; Bcenmc03_0119ATP synthase subunit delta; ATP synthase F(1) sector subunit delta; F-type ATPase subunit delta; F-ATPase subunit delta
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT45819
Source
E.coli
Synonyms
atpH; Bcenmc03_0119ATP synthase subunit delta; ATP synthase F(1) sector subunit delta; F-type ATPase subunit delta; F-ATPase subunit delta
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT45819
Source
Baculovirus
Synonyms
atpH; Bcenmc03_0119ATP synthase subunit delta; ATP synthase F(1) sector subunit delta; F-type ATPase subunit delta; F-ATPase subunit delta
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT45819
Source
Mammalian cell
Synonyms
atpH; Bcenmc03_0119ATP synthase subunit delta; ATP synthase F(1) sector subunit delta; F-type ATPase subunit delta; F-ATPase subunit delta
Purity
>85% (SDS-PAGE)
Quick Inquiry

Description

Overview of ATP Synthase in Burkholderia cenocepacia

ATP synthase is a critical enzyme complex for cellular energy production, catalyzing ATP synthesis via oxidative phosphorylation. In Burkholderia cenocepacia, ATP synthase consists of multiple subunits, including the delta (δ) subunit encoded by the atpH gene. This subunit stabilizes the F1 portion of ATP synthase, ensuring efficient coupling of proton translocation and ATP synthesis .

Key Features of ATP Synthase Subunit Delta (atpH):

  • Role: Structural stabilization of the F1 complex, ensuring rotational catalysis efficiency.

  • Gene: atpH (BCAM_xxxx, locus-specific identifier varies by strain).

  • Post-Translational Modifications: Potential phosphorylation sites inferred from homologous bacterial systems .

Recombinant Production and Purification

Recombinant B. cenocepacia ATP synthase subunit delta is produced through heterologous expression systems, typically in Escherichia coli. Cloning involves PCR amplification of atpH followed by ligation into expression vectors (e.g., pET23a+) and transformation into BL21(DE3) cells .

Example Protocol:

StepDetails
Gene AmplificationPrimers designed with NdeI/XhoI restriction sites .
Vector CloningLigation into pET23a+ for His-tag fusion .
Induction0.5 mM IPTG at OD<sub>600</sub> = 0.6, 18°C for 16 hr .
PurificationNi-NTA affinity chromatography under denaturing conditions .

Yield: ~15–20 mg/L culture, purity >95% (SDS-PAGE verified) .

Research Applications and Challenges

Applications:

  • Antimicrobial Target Studies: ATP synthase is a target for diarylquinolines (e.g., bedaquiline) .

  • Structural Biology: Cryo-EM studies to map drug-binding sites .

Challenges in Study:

  • Membrane Localization: Requires detergent solubilization for in vitro assays .

  • Regulatory Complexity: Co-expression with other subunits (e.g., α, β, γ) is often necessary for functional reconstitution .

Future Directions

  1. CRISPRi-Based Knockdown: Utilize CRISPR interference (as in ) to study atpH essentiality in B. cenocepacia biofilms.

  2. Proteomic Profiling: Combine surfomics (surface-shaving LC-MS/MS ) with δ-subunit immunoprecipitation to map interactors.

  3. Therapeutic Development: Screen δ-subunit inhibitors using thermal shift assays .

Critical Gaps in Current Knowledge

  • No direct structural or enzymatic data on B. cenocepacia δ subunit exists in the literature reviewed.

  • Role in antimicrobial resistance (e.g., RND efflux pumps ) remains unexplored.

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format, but special format requests can be made during ordering.
Lead Time
Delivery times vary based on purchasing method and location. Consult local distributors for specific delivery times. Proteins are shipped with blue ice packs by default; dry ice shipping is available upon request for an extra fee.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us and we will prioritize its development.
Synonyms
atpH; Bcenmc03_0119ATP synthase subunit delta; ATP synthase F(1) sector subunit delta; F-type ATPase subunit delta; F-ATPase subunit delta
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-179
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Burkholderia cenocepacia (strain MC0-3)
Target Names
atpH
Target Protein Sequence
MAELATIARP YAEALFRVAE GGDIAAWSTL VQELAQVARL PEVLSVASSP KVTRTQVAEL LLAAVKSPVA AGAEAKNFVQ MLVDNHRIAL LPEIAEQFEA LKNEREGAAD AEIVSAFPLN GADLDSLVSG LERKFKRKLK PTVEVDSSLI GGVRVTVGDE VLDTSVRARL ASMQAALTA
Uniprot No.
B1JSV4

Target Background

Function
F(1)F(0) ATP synthase synthesizes ATP from ADP using a proton or sodium gradient. It comprises two domains: F(1) (catalytic core) and F(0) (membrane proton channel), connected by a central and peripheral stalk. ATP synthesis in F(1) is coupled to proton translocation in F(0) via a rotary mechanism. This protein is a stalk component linking CF(0) to CF(1), involved in either transmitting conformational changes or proton conduction.
Database Links

KEGG: bcm:Bcenmc03_0119

Protein Families
ATPase delta chain family
Subcellular Location
Cell inner membrane; Peripheral membrane protein.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2025 TheBiotek. All Rights Reserved.