Recombinant Burkholderia cenocepacia L-threonine 3-dehydrogenase (tdh)

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Cat. No.
BT39097
Source
Yeast
Synonyms
tdh; Bcenmc03_4535; L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39097
Source
E.coli
Synonyms
tdh; Bcenmc03_4535; L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39097
Source
E.coli
Synonyms
tdh; Bcenmc03_4535; L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39097
Source
Baculovirus
Synonyms
tdh; Bcenmc03_4535; L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39097
Source
Mammalian cell
Synonyms
tdh; Bcenmc03_4535; L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103
Purity
>85% (SDS-PAGE)
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Description

Biochemical Properties and Function

The tdh enzyme belongs to the medium-chain dehydrogenase/reductase (MDR) superfamily, sharing structural and functional homology with alcohol dehydrogenases. Its primary role is to oxidize L-threonine, an essential amino acid, into a ketobutyrate intermediate, which contributes to glycine biosynthesis and redox balance . In Burkholderia species, this pathway is linked to metabolic flexibility and stress adaptation, as evidenced by its upregulation under nutrient-limiting conditions .

PropertyValue
Molecular Weight~40 kDa (monomer); ~160 kDa (tetramer)
Optimal pH7.4–8.5
Coenzyme RequirementNAD⁺
Substrate SpecificityL-threonine (Km ≈ 0.5 mM)
InhibitorsPyruvate (competitive), Antabuse (parasite-specific)

3.1. Role in Burkholderia Pathogenesis

  • Survival in Hosts: A transposon mutagenesis study identified tdh (Bcep1341) as essential for Burkholderia cenocepacia’s survival in rat lung infections, highlighting its role in metabolic adaptation .

  • Biofilm Formation: The enzyme is linked to biofilm architecture and stress resistance, with mutants showing reduced motility and altered cyclic-di-GMP levels .

  • Vaccine Targets: Surface-exposed tdh homologs (e.g., BCAL2645) have been proposed as immunogenic candidates for Burkholderia vaccines .

3.2. Recombinant Production

Recombinant tdh from Burkholderia vietnamiensis (strain G4) has been expressed in E. coli, yielding >85% pure protein via SDS-PAGE . Its sequence (Uniprot A4JKG7) aligns with cenocepacia homologs, suggesting functional conservation .

Recombinant Product DetailsValue
Product CodeCSB-BP023350BPV/CSB-MP023350BPV
Host OrganismE. coli
Purity>85% (SDS-PAGE)
Storage-20°C/-80°C

Applications and Implications

  • Diagnostic Tools: Recombinant tdh may serve as a target for serological assays to detect Burkholderia infections in cystic fibrosis patients .

  • Therapeutic Targets: Inhibitors of tdh could disrupt glycine biosynthesis, offering a novel antimicrobial strategy .

  • Biotechnology: The enzyme’s substrate promiscuity and thermostability make it suitable for industrial biocatalysis .

Product Specs

Form
Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering.
Lead Time
Delivery time varies by purchasing method and location. Consult local distributors for specific delivery times. Proteins are shipped with blue ice packs by default. Request dry ice in advance for an extra fee.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Default glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us and we will prioritize its development.
Synonyms
tdh; Bcenmc03_4535; L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-342
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Burkholderia cenocepacia (strain MC0-3)
Target Names
tdh
Target Protein Sequence
MKALAKLERG PGLTLTRVKR PEVGHNDVLI KIRRTAICGT DIHIWKWDDW AQKTIPVPMH VGHEYVGEIV EMGQEVRGFA IGDRVSGEGH ITCGFCRNCR AGRRHLCRNT VGVGVNREGA FAEYLAIPAF NAFKIPPEIS DDLASIFDPF GNATHTALSF NLVGEDVLIT GAGPIGIMAV AIAKHVGARN VVITDINDYR LELARKMGAT RAVNVARESL RDVMADLHMT EGFDVGLEMS GVPSAFTSLL EAMNHGGKVA LLGIPPAQTA IDWNQVIFKG LEIKGIYGRE MFETWYKMVA MLQSGLDLSP IITHRFAADD YEQGFAAMLS GESGKVILDW TA
Uniprot No.
B1K269

Target Background

Function
Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate.
Database Links

KEGG: bcm:Bcenmc03_4535

Protein Families
Zinc-containing alcohol dehydrogenase family
Subcellular Location
Cytoplasm.

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