Recombinant Burkholderia multivorans ATP phosphoribosyltransferase (hisG)
Description
Burkholderia multivorans
Burkholderia multivorans is a bacterium within the Burkholderia cepacia complex (Bcc), known for causing opportunistic lung infections, especially in individuals with cystic fibrosis (CF) . B. multivorans is typically acquired from environmental sources, with rare instances of patient-to-patient transmission . Genomic analysis reveals high strain diversity between patients, suggesting an environmental microdiverse reservoir where active diversification occurs . Burkholderia species exhibit diverse metabolic activities and can be both beneficial and pathogenic .
ATP Phosphoribosyltransferase (hisG) in Burkholderia multivorans
The hisG gene encodes ATP phosphoribosyltransferase, a critical enzyme in the histidine biosynthesis pathway. While the provided data does not explicitly detail the recombinant form of Burkholderia multivorans ATP phosphoribosyltransferase (hisG), understanding its role in histidine biosynthesis and its presence in Burkholderia species is crucial. The Burkholderia species exhibits a wide array of specialized metabolites, including non-ribosomal peptides, which suggests a diverse genetic capacity .
Role in Histidine Biosynthesis
ATP phosphoribosyltransferase (HisG) plays a vital role in regulating histidine biosynthesis. Histidine is an essential amino acid required for protein synthesis and various metabolic processes. The hisG gene product catalyzes the initial regulatory step in this pathway.
Potential Research Directions
Further research could explore:
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The specific characteristics of recombinant Burkholderia multivorans ATP phosphoribosyltransferase (hisG), including its structure, function, and regulation.
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The role of histidine biosynthesis in the adaptation and survival of B. multivorans within the CF lung environment.
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The potential for targeting HisG as an antibacterial strategy against B. multivorans infections.
Tables
Because the request specified the inclusion of data tables, I have provided representative tables from the documents found that are related to the topic.
Table 1: Prevalence of Specialized Metabolite Classes Across Burkholderia Sensu Lato Genera
| Specialized Metabolite Class | Burkholderia (% Species) | Paraburkholderia (% Species) | Mycetohabitans (% Species) | Caballeronia (% Species) | Robbsia (% Species) | Trinickia (% Species) | Pararobbsia (% Species) |
|---|---|---|---|---|---|---|---|
| Terpene | 100 | 100 | 100 | 100 | 100 | 100 | N/A |
| NRPS | 96 | 100 | 100 | 80 | 80 | 80 | N/A |
| Phosphonate | 92 | 97 | 100 | 60 | 100 | 100 | N/A |
| Aryl Polyene | 100 | 100 | 0 | 100 | 100 | 100 | N/A |
| Homoserine Lactone | 100 | 97 | N/A | 20 | 100 | 100 | N/A |
| Lassopeptide | 28 | 3 | 100 | 0 | 0 | 3 | N/A |
| Trans-AT-PKS | 6 | 2 | 33 | 20 | 0 | 0 | N/A |
This table illustrates the variation in specialized metabolite classes across different genera within Burkholderia sensu lato .
Table 2: Loci with mutations (SNPs) in at least three patients infected with B. multivorans
Due to the length of the original table, a representative excerpt is shown. For a comprehensive view, refer to the original source .
| Locus | Gene | Product | Patient 1 | Patient 2 (2011) | Patient 2 (2018) | Patient 3 | Patient 4 |
|---|---|---|---|---|---|---|---|
| 129 | pgm | Phosphoglucomutase | Yes | Yes | Yes | Yes | Yes |
| 6144 | dnaK | Chaperone protein DnaK | Yes | Yes | Yes | Yes | Yes |
| 4498 | - | Hypothetical protein | Yes | Yes | - | Yes | Yes |
| 5106 | oppA | Periplasmic oligopeptide-binding protein | Yes | Yes | - | Yes | - |
Product Specs
Note: We will prioritize shipment of the format currently in stock. However, if you require a specific format, please specify this during order placement. We will fulfill your request to the best of our ability.
Note: Our proteins are shipped standard with blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C. Lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
The tag type is determined during the production process. If you require a specific tag type, please inform us, and we will prioritize its development.
Target Background
ATP phosphoribosyltransferase (HisG) catalyzes the condensation of ATP and 5-phosphoribosyl 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). HisG plays a crucial role in histidine biosynthesis, primarily regulating the pathway's rate.
KEGG: bmj:BMULJ_02927
STRING: 395019.BMULJ_02927
