Recombinant Callithrix jacchus Septin-8 (SEPT8)
Description
Overview of Septin-8 (SEPT8)
SEPT8 is a GTPase belonging to the septin family, which forms filamentous scaffolds involved in:
In humans, SEPT8 interacts with proteins like SEPT5, VAMP2, and syntaxin1A, influencing neurotransmitter release and platelet secretion . It also localizes to cilia and actin-rich regions under stress .
Recombinant SEPT8 in Research
Recombinant SEPT8 is typically produced in systems like E. coli or mammalian cells for functional studies. While no direct data exists for Callithrix jacchus SEPT8, human and rodent homologs provide foundational insights:
Disease Associations
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Kidney Injury: Elevated SEPT8 correlates with tubulointerstitial damage and proteinuria in rats .
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Platelet Function: SEPT8 translocates to platelet surfaces upon activation, influencing secretion .
Antibody and Detection Tools
Though not validated for Callithrix jacchus, cross-reactive antibodies (e.g., Proteintech 11769-1-AP) detect SEPT8 in humans, mice, and rats :
| Application | Recommended Dilution | Detected In |
|---|---|---|
| Western Blot (WB) | 1:2000–1:10,000 | HeLa cells, brain tissue |
| Immunofluorescence | 1:50–1:500 | Co-localizes with tubulin |
Current Gaps and Future Directions
No peer-reviewed studies specifically address Recombinant Callithrix jacchus SEPT8. Key unknowns include:
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Sequence Conservation: Marmoset vs. human/rodent SEPT8.
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Functional Divergence: Unique roles in primate-specific pathways.
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Experimental Models: Utility in neurological or renal studies.
Product Specs
Target Background
STRING: 9483.ENSCJAP00000027173
