Recombinant Campylobacter fetus subsp. fetus ATP synthase subunit a (atpB)
Description
Molecular Characterization of Recombinant ATP Synthase Subunit a (atpB)
Gene and Protein Structure
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Encoded by the atpB gene (locus tag: CFF8240_0771), this subunit is part of the F0 sector of the ATP synthase complex, which drives ATP synthesis via proton translocation .
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The recombinant protein spans 226 amino acids (UniProt ID: A0RP13) and includes an N-terminal His tag for purification .
Functional Role
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Essential for oxidative phosphorylation: Facilitates proton transport across the membrane, enabling ATP synthesis .
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Structural analyses reveal conserved transmembrane helices critical for interactions with other F0 subunits (e.g., subunit c) .
Production and Purification
Expression System
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Produced in Escherichia coli using plasmid vectors optimized for Campylobacter-specific codon usage .
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Purification involves immobilized metal affinity chromatography (IMAC) via the His tag, yielding >90% purity .
Antigenic Studies
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Used in ELISA to detect C. fetus-specific antibodies in clinical samples .
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Immunogenicity studies suggest potential as a diagnostic marker for bovine and human infections .
Functional Genomics
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Proteomic analyses (e.g., LFQ-MS) reveal altered expression in C. fetus glycosylation mutants, linking ATP synthase activity to hydrogenase regulation and nickel homeostasis .
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Comparative genomics identifies atpB as a stable genetic marker for subspecies differentiation (e.g., C. fetus subsp. fetus vs. venerealis) .
Genomic and Phenotypic Correlations
Subspecies-Specific Features
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atpB is conserved across C. fetus subspecies but exhibits transcriptional differences in niche-adapted strains .
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In C. fetus subsp. venerealis, ATP synthase activity correlates with enhanced virulence gene expression via a type IV secretion system (T4SS) .
Antimicrobial Resistance (AMR)
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No direct AMR linkage reported, but C. fetus subsp. fetus harbors plasmid-borne resistance genes (e.g., tet(O), aph(3')-III) .
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Recombinant atpB serves as a control in studies assessing AMR-related metabolic shifts .
Future Directions
Product Specs
Please note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please specify them in your order notes, and we will fulfill your request if possible.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance, as additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: cff:CFF8240_0771
