Recombinant Campylobacter fetus subsp. fetus Queuine tRNA-ribosyltransferase (tgt)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder. We will preferentially ship the format we have in stock. If you have special format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with blue ice packs by default. Request dry ice in advance (extra fees apply).
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer ingredients, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
tgt; CFF8240_0980Queuine tRNA-ribosyltransferase; EC 2.4.2.29; Guanine insertion enzyme; tRNA-guanine transglycosylase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-374
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Campylobacter fetus subsp. fetus (strain 82-40)
Target Names
tgt
Target Protein Sequence
MNFKIDKTDG NARACTLQTA HSTIQTPIFM PVGTLGAVKS LDAIDLKEIL DAKIILANTY
HLYLRPTSKV VREFGGLHGF SKFDRSFLTD SGGFQAFSLS KISKPDENGI KFKSHIDGSM
HYFTPKSVLD TQYDLSSDIM MILDDLVALP ATKERIDLSI KRTINWAKIA CEYHKSNKQK
SVGIDQNIFG IIQGGTDYNA RKLCAEALCE MEFDGLAIGG LSVGESNEEM YDTVEALMPF
IDKNRPRYLM GVGTPEDLVQ NVERGVDMFD CVMPTRNARN GTLFTSFGKI NIKSAAFIKD
DNKIDPECDC YTCSNFSRGY LNHLYKAREL TFFRLASLHN LHYYLNLVKQ MREAIMQGKF
KEFKREFYAK RGMI
Uniprot No.
Target Background
Function
Catalyzes the base-exchange of guanine (G) with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His, and -Tyr). This occurs via a double-displacement mechanism. The active site attacks the C1' of nucleotide 34, detaching the guanine base and forming a covalent enzyme-RNA intermediate. PreQ1 is deprotonated, allowing it to attack the C1' of the ribose to form the product. After dissociation, two further enzymatic reactions convert PreQ1 to queuine (Q), resulting in queuosine.
Database Links
KEGG: cff:CFF8240_0980
Protein Families
Queuine tRNA-ribosyltransferase family
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