Recombinant Campylobacter jejuni subsp. doylei Undecaprenyl-diphosphatase (uppP)
Description
Introduction to Recombinant Campylobacter jejuni subsp. doylei Undecaprenyl-diphosphatase (uppP)
Recombinant Campylobacter jejuni subsp. doylei Undecaprenyl-diphosphatase (uppP) is a bacterially derived enzyme produced through recombinant DNA technology. This protein, encoded by the uppP gene (locus JJD26997_0215 in strain ATCC BAA-1458/RM4099/269.97), catalyzes the hydrolysis of undecaprenyl diphosphate (UPP) to undecaprenyl phosphate (UP), a critical step in bacterial cell wall biosynthesis . Its role in lipid carrier regeneration makes it a target for studying antibiotic resistance mechanisms, particularly bacitracin resistance .
Protein Features
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Amino Acid Sequence: Partial sequence (residues 1–267) with predicted domains for phosphatase activity .
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Expression Systems: Produced in E. coli, baculovirus, or mammalian cells .
Antibiotic Resistance Studies
uppP confers bacitracin resistance by recycling UP, a lipid carrier essential for peptidoglycan synthesis. Disruption of uppP activity sensitizes bacteria to bacitracin, highlighting its role as a drug target .
Pathogenicity and Vaccine Development
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Host Interaction: uppP contributes to C. jejuni survival in host environments, including poultry reservoirs .
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Recombinant Use: The protein is utilized in ELISA and immunoblot assays to study immune responses during Campylobacter infections .
Evolutionary and Genomic Context
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Genetic Diversity: The uppP gene is conserved across C. jejuni strains but undergoes rapid evolution via recombination, as observed in C. jejuni subsp. doylei .
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Horizontal Gene Transfer: Recombination rates in Campylobacter spp. are twice the mutation rate, facilitating interspecies gene flow (e.g., with C. coli) .
Practical Considerations for Use
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Reconstitution: Lyophilized protein should be dissolved in sterile water to 0.1–1.0 mg/mL, with glycerol (5–50%) added for long-term stability .
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Limitations: Avoid repeated freeze-thaw cycles; working aliquots stored at 4°C retain activity for ≤1 week .
Future Directions
Current research gaps include direct functional assays for uppP in C. jejuni subsp. doylei and structural studies to guide inhibitor design. The protein’s role in zoonotic transmission and antibiotic resistance warrants further exploration .
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please specify them in your order notes, and we will prepare the product accordingly.
Note: All our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: cjd:JJD26997_0215
