Recombinant Candida albicans NADH-cytochrome b5 reductase 2 (MCR1)
Description
Functional Roles
MCR1 participates in:
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Ergosterol biosynthesis: Electron transfer to cytochrome b5 for sterol desaturation .
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Oxidative stress response: Overexpression enhances resistance to hydrolysate inhibitors (e.g., furfural) by accelerating furaldehyde reduction .
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Fatty acid metabolism: Supports PUFA biosynthesis in M. alpina via NADH-dependent electron transport .
Recombinant Expression and Purification
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Expression systems: Successfully expressed in E. coli (limited solubility) and Pichia pastoris .
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Purification methods:
Table 2: Enzymatic Activity of Recombinant MCR1 Homologs
Research Implications
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Biotechnological applications: Engineered S. cerevisiae strains with MCR1 overexpression show improved ethanol production rates in lignocellulose hydrolysates .
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Mitochondrial dynamics: Incomplete outer membrane arrest sorts MCR1 to dual submitochondrial compartments, suggesting regulatory crosstalk between translocation machineries .
Knowledge Gaps
No direct studies on Candida albicans MCR1 exist in the reviewed literature. Current insights derive from homologous systems, highlighting the need for C. albicans-specific investigations to resolve its role in pathogenicity or antifungal resistance.
Product Specs
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Target Background
KEGG: cal:CAALFM_C602040WA
