Recombinant Candida glabrata Nascent polypeptide-associated complex subunit beta (EGD1)

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Cat. No.
BT2449994
Source
Yeast
Synonyms
EGD1; CAGL0L12540g; Nascent polypeptide-associated complex subunit beta; NAC-beta; Beta-NAC
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2449994
Source
E.coli
Synonyms
EGD1; CAGL0L12540g; Nascent polypeptide-associated complex subunit beta; NAC-beta; Beta-NAC
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2449994
Source
E.coli
Synonyms
EGD1; CAGL0L12540g; Nascent polypeptide-associated complex subunit beta; NAC-beta; Beta-NAC
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2449994
Source
Baculovirus
Synonyms
EGD1; CAGL0L12540g; Nascent polypeptide-associated complex subunit beta; NAC-beta; Beta-NAC
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2449994
Source
Mammalian cell
Synonyms
EGD1; CAGL0L12540g; Nascent polypeptide-associated complex subunit beta; NAC-beta; Beta-NAC
Purity
>85% (SDS-PAGE)
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Description

Protein Biogenesis and Mitochondrial Quality Control

EGD1 facilitates nascent polypeptide chain binding to ribosomes and coordinates protein targeting to mitochondria. Key findings include:

  • Mitophagy Regulation: EGD1 interacts with Atg32, a pro-mitophagic protein, to enable mitochondrial degradation. Deletion of EGD1 reduces Atg32 phosphorylation and mitophagy efficiency, whereas BTT1 (a paralogous β-subunit) deletion has minimal impact .

  • Protein Folding: NAC disruption alters chaperone balance, rescuing polyglutamine-induced toxicity in yeast. EGD1Δ strains show partial rescue of htt-103Q toxicity, unlike EGD2Δ or BTT1Δ strains .

Transcriptional Regulation

EGD1 influences transcriptional activation in galactose-regulated genes. Disruption of EGD1 delays GAL1/10 induction during carbon source shifts, suggesting a role in modulating Gal4p activity .

Experimental Models

ApplicationDescriptionSource
ELISADetection of EGD1-specific antibodies in immune assays
Disease ModelingStudying mitochondrial dysfunction in neurodegenerative diseases (e.g., Huntington’s) ,
Protein InteractionMapping interactions with Atg32 and mitochondrial import machinery

Genetic Insights from Deletion Studies

  • Mitochondrial Degradation: EGD1Δ yeast exhibit impaired mitophagy and reduced Atg32 phosphorylation .

  • Transcriptional Defects: EGD1 disruption delays GAL1/10 RNA accumulation during galactose induction .

Comparative Analysis of EGD1 and BTT1

EGD1 and BTT1 are paralogous β-subunits with distinct roles:

FeatureEGD1BTT1Source
Cellular ConcentrationDominant β-subunit (100-fold higher)Minor β-subunit
Mitophagy ImpactEssential for Atg32 phosphorylationPartial reduction in mitophagy
Protein StabilityStabilizes NAC-ribosome interactionLess critical for NAC function

Future Directions and Clinical Relevance

EGD1’s role in mitochondrial quality control and transcriptional regulation positions it as a potential therapeutic target for diseases involving protein misfolding or metabolic dysfunction. Further studies could explore:

  • Antifungal Strategies: Leveraging EGD1’s interaction with Atg32 to enhance antifungal efficacy.

  • Neurodegenerative Diseases: Investigating EGD1’s role in mitigating polyglutamine toxicity in mammalian models.

Product Specs

Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested in advance. Additional fees apply for dry ice shipping.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a reference.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer components, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize development accordingly.
Synonyms
EGD1; CAGL0L12540g; Nascent polypeptide-associated complex subunit beta; NAC-beta; Beta-NAC
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-156
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Target Names
EGD1
Target Protein Sequence
MPIDQEKLAK LQKMSAGNKV GGTRRKQAKK TGSGSAGNKD DTKLHNQLAK LHAVTIDNVA EANFFKDDGK VLHFNKVGVQ VAPQHNTSVF YGMAQEKNLQ ELFPGIISQL GGEAIQALSQ LAAQMEKAQA NENAGEAKDE AIPELVEGQS FDAEVE
Uniprot No.
Q6FKD1

Target Background

Function
Recombinant *Candida glabrata* Nascent polypeptide-associated complex subunit beta (EGD1) is a component of the nascent polypeptide-associated complex (NAC). NAC dynamically resides within the ribosomal exit tunnel, shielding nascent polypeptides from cytoplasmic interactions to ensure proper protein targeting. Additionally, NAC facilitates mitochondrial protein import by optimizing ribosome interactions with the outer mitochondrial membrane and preventing inappropriate interactions between ribosomes translating non-secretory nascent polypeptides and endoplasmic reticulum translocation sites. EGD1 may function as a transcription factor, negatively regulating the expression of various RNA polymerase II-transcribed genes.
Database Links

KEGG: cgr:CAGL0L12540g

STRING: 284593.XP_449313.1

Protein Families
NAC-beta family
Subcellular Location
Cytoplasm. Nucleus.

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