Recombinant Candida glabrata RNA polymerase II transcription factor B subunit 3 (TFB3)
Description
Overview of RNA Polymerase II Transcription Factors in C. glabrata
RNA polymerase II (RNAPII) transcription in fungi requires general transcription factors (GTFs), including TFIIB, TFIIH, and Mediator complexes. While TFIIB is a single-subunit GTF, TFIIH is a multi-subunit complex essential for transcription initiation and DNA repair. In Saccharomyces cerevisiae, TFIIH comprises core subunits (e.g., Tfb1, Tfb2, Ssl1, Tfb4, Tfb5) and the CDK-activating kinase (CAK) module (Kin28, Ccl1, Tfb3/Mat1). Homologs of these subunits are likely conserved in C. glabrata, though nomenclature and functional studies vary .
Key Components of TFIIH in Yeast:
| Subunit (S. cerevisiae) | Homolog in C. glabrata | Role |
|---|---|---|
| Tfb1 (p62) | Likely conserved | Core TFIIH stability |
| Tfb2 (p52) | Likely conserved | Helicase coordination |
| Tfb3 (Mat1) | Putative homolog | CAK module stability |
Functional Context of TFB3
In S. cerevisiae, Tfb3 (Mat1) stabilizes the CAK module (Kin28-Ccl1-Tfb3), which phosphorylates RNAPII’s C-terminal domain (CTD) to enable transcriptional elongation . While C. glabrata TFB3 remains uncharacterized, studies on related GTFs provide insights:
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TFIIH Mutagenesis: Systematic mutagenesis of S. cerevisiae Tfb2 revealed functional interactions with Tfb3/Mat1, where CAK module integrity impacts RNAPII recruitment and promoter occupancy .
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Drug Resistance: Disruption of TFIIH subunits in C. glabrata (e.g., via calcineurin signaling) affects stress responses and antifungal resistance .
Research Gaps and Indirect Evidence
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Mediator Complex: Subunits like CgMed3AB regulate acetyl-CoA metabolism and cell growth, indirectly influencing transcriptional activity .
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Transcriptional Adaptations: RNAPII occupancy profiling during macrophage infection reveals dynamic gene expression tied to stress responses and azole tolerance .
Comparative Genomics and Evolutionary Insights
C. glabrata exhibits microevolutionary changes in transcriptional regulators (e.g., ERG4, FKS1/2) that alter antifungal resistance . While TFB3-specific data are absent, conserved TFIIH functions suggest its role in maintaining transcriptional fidelity under stress.
Future Directions
Further studies should:
Product Specs
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Target Background
KEGG: cgr:CAGL0K06369g
STRING: 284593.XP_448500.1
