Recombinant Candida glabrata Signal recognition particle SEC65 subunit (SEC65)

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Cat. No.
BT2435313
Source
Yeast
Synonyms
SEC65; CAGL0J06578g; Signal recognition particle SEC65 subunit
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2435313
Source
E.coli
Synonyms
SEC65; CAGL0J06578g; Signal recognition particle SEC65 subunit
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2435313
Source
E.coli
Synonyms
SEC65; CAGL0J06578g; Signal recognition particle SEC65 subunit
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2435313
Source
Baculovirus
Synonyms
SEC65; CAGL0J06578g; Signal recognition particle SEC65 subunit
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2435313
Source
Mammalian cell
Synonyms
SEC65; CAGL0J06578g; Signal recognition particle SEC65 subunit
Purity
>85% (SDS-PAGE)
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Description

Introduction to Recombinant Candida glabrata SEC65 Subunit

The recombinant Candida glabrata Signal Recognition Particle (SRP) SEC65 subunit (SEC65) is a genetically engineered protein derived from the CAGL0J06578g gene. It serves as a critical component of the SRP complex, which mediates co-translational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER) in eukaryotes . SEC65 homologs are conserved across fungi, including Saccharomyces cerevisiae and Candida albicans, sharing structural and functional similarities with mammalian SRP19 .

Genomic Data

  • Gene Locus: CAGL0J06578g is located on chromosome J in C. glabrata.

  • Orthologs:

    • S. cerevisiae: SEC65 (YML105C)

    • C. albicans: SEC65 (orf19.6443)

    • Schizosaccharomyces pombe: sec65 (SPCC126.15c)

Proteomic Interactions

The SRP complex in C. glabrata likely mirrors the S. cerevisiae SRP structure, which includes:

  1. SRP54: Signal sequence-binding subunit.

  2. scR1 RNA: Structural scaffold.

  3. SEC65: Stabilizes SRP54 association with scR1 .

Production Platforms

Host SystemPurity (SDS-PAGE)ApplicationsSource
E. coli≥85%ELISA, Western blotting
Yeast≥85%Structural studies, functional assays
Baculovirus≥85%Large-scale production for biotech

Recombinant SEC65 is purified for use in:

  • Biochemical assays: Studying SRP assembly dynamics.

  • Diagnostic tools: Antibody development (e.g., rabbit anti-SEC65 polyclonal antibodies) .

  • Therapeutic research: Exploring SRP-targeted antifungal strategies .

Key Discoveries

  1. SRP Assembly Dependency:

    • SEC65 is essential for SRP54’s stable binding to scR1 RNA in S. cerevisiae .

    • sec65-1 mutants exhibit temperature-sensitive growth defects and impaired membrane protein integration .

  2. Suppression Mechanisms:

    • Overexpression of SRP54 partially restores SRP function in sec65-1 mutants, suggesting compensatory interactions .

  3. Clinical Relevance:

    • SRP dysfunction may impact C. glabrata’s ability to produce virulence factors (e.g., adhesins, proteases) due to disrupted protein secretion .

    • Genome-wide studies of C. glabrata clinical isolates highlight genetic diversity in SRP-related genes, potentially influencing antifungal resistance .

Product Specs

Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchase method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, offered as a guideline.
Shelf Life
Shelf life depends on storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type is determined during production. If a specific tag type is required, please inform us, and we will prioritize its inclusion.
Synonyms
SEC65; CAGL0J06578g; Signal recognition particle SEC65 subunit
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-264
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Target Names
SEC65
Target Protein Sequence
MPKLEEIEDY DDIDNMDMDL AELDPTLNTP IAPKITPTVV RSQDIEEEER KKQYAGSNKE MISFINPQTG KVEESAPITK QELDEVKKFQ VLYPCYFDKN RSHKEGRRVP IEMAVENPLA KTISDAVREY GLLSIFEGDK CHPQDFGNPG RIRVLLKEEG KLVPSASKFS GGKRQLMKLV AEYLQKHPTT VESLRELPYG PDFDNIEFKK IPKVKGFKMN DIVPLHSPYL WGHPMTKSIY EAPKIEAPEK QFKAPKNKFK VVRR
Uniprot No.
Q6FP52

Target Background

Function

Signal recognition particle (SRP) assembly plays a critical role in targeting secretory proteins to the rough endoplasmic reticulum (RER) membrane. Its involvement is essential for the translocation of a diverse range of protein substrates.

Database Links

KEGG: cgr:CAGL0J06578g

STRING: 284593.XP_447992.1

Protein Families
SRP19 family
Subcellular Location
Cytoplasm.

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