Recombinant Capparis masaikai Sweet protein mabinlin-4

Shipped with Ice Packs
In Stock
Cat. No.
BT36579
Source
Yeast
Synonyms
Sweet protein mabinlin-4; Mabinlin IV; MAB IV) [Cleaved into: Sweet protein mabinlin-4 chain A; Sweet protein mabinlin-4 chain B]
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT36579
Source
E.coli
Synonyms
Sweet protein mabinlin-4; Mabinlin IV; MAB IV) [Cleaved into: Sweet protein mabinlin-4 chain A; Sweet protein mabinlin-4 chain B]
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT36579
Source
E.coli
Synonyms
Sweet protein mabinlin-4; Mabinlin IV; MAB IV) [Cleaved into: Sweet protein mabinlin-4 chain A; Sweet protein mabinlin-4 chain B]
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT36579
Source
Baculovirus
Synonyms
Sweet protein mabinlin-4; Mabinlin IV; MAB IV) [Cleaved into: Sweet protein mabinlin-4 chain A; Sweet protein mabinlin-4 chain B]
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT36579
Source
Mammalian cell
Synonyms
Sweet protein mabinlin-4; Mabinlin IV; MAB IV) [Cleaved into: Sweet protein mabinlin-4 chain A; Sweet protein mabinlin-4 chain B]
Purity
>85% (SDS-PAGE)
Quick Inquiry

Description

Recombinant Production

While no direct studies on recombinant Mabinlin-4 exist, its production likely parallels methods developed for Mabinlin-II:

  • E. coli systems: Expression in inclusion bodies followed by denaturation/renaturation .

  • Food-grade hosts: Lactococcus lactis for secretion, validated for Mabinlin-II .

Challenges:

  • Mabinlin-4’s shorter molecular weight (11.9 kDa vs. 12.3 kDa for Mabinlin-1/3) may affect folding efficiency .

  • The absence of arginine at position 47 in the B-chain (present in heat-stable Mabinlin-II) suggests lower thermal stability .

Functional Properties

  • Sweetness: Mabinlin-4’s sweetness has not been quantified, but Mabinlin-II is reported to be ~400x sweeter than sucrose .

  • Taste receptor interaction: The B-chain’s [NL/I] tetralet motif (critical for sweetness in Mabinlin-II) is conserved in Mabinlin-4’s B-chain .

Comparative Analysis

FeatureMabinlin-4Mabinlin-2
Amino acid identity95% (B-chain)90% (B-chain)
Disulfide bridges4 (2 interchain, 2 intrachain)4 (2 interchain, 2 intrachain)
Expression yieldNot reported2.5-fold increase at 25°C

Research Gaps

  • Sweetness quantification: No direct measurements of Mabinlin-4’s sweetness relative to sucrose or other homologues.

  • Thermostability: Unstudied, though structural homology suggests lower stability than Mabinlin-2.

  • Recombinant optimization: No tailored expression systems for Mabinlin-4 have been reported.

Product Specs

Form
Lyophilized powder. We will ship the in-stock format preferentially. If you have special format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. Request dry ice shipping in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us and we will prioritize its development.
Synonyms
Sweet protein mabinlin-4; Mabinlin IV; MAB IV) [Cleaved into: Sweet protein mabinlin-4 chain A; Sweet protein mabinlin-4 chain B]
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-28
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Capparis masaikai (Mabinlang)
Target Protein Sequence
EPLCRRQFQQ HQHLRACQRY LRRRAQRG
Uniprot No.
P80353

Target Background

Function
Heat-stable 2S seed storage protein with sweetness-inducing activity.
Protein Families
2S seed storage albumins family

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2025 TheBiotek. All Rights Reserved.