Recombinant Cervus nippon pseudaxis Cytochrome b (MT-CYB) is a recombinant protein derived from the Tonkin sika deer, a subspecies of the sika deer (Cervus nippon). This protein is a crucial component of the mitochondrial electron transport chain, specifically part of Complex III, also known as the ubiquinol-cytochrome c reductase complex. The cytochrome b subunit plays a pivotal role in energy production and is essential for cellular respiration.
Cytochrome b is a hydrophobic protein consisting of eight transmembrane helices. It contains two hemes and forms the ubiquinol and inhibitor binding sites, known as Qo and Qi sites, which are critical for the electron transport process . This protein is encoded by mitochondrial DNA (mtDNA) and is involved in generating a proton gradient across the mitochondrial inner membrane, which drives ATP synthesis.
The recombinant Cervus nippon pseudaxis Cytochrome b (MT-CYB) is produced in mammalian cells, ensuring high purity (>85% as determined by SDS-PAGE) and biological activity . The protein is available in both liquid and lyophilized forms, with shelf lives of 6 months and 12 months, respectively, when stored at -20°C/-80°C .