Recombinant Chicken Frizzled-1 (FZD1)
Description
Detection and Quantification
The Chicken Frizzled-1 ELISA Kit (Assay Genie) is a widely used tool for measuring FZD1 levels in biological samples. Key specifications include:
| Parameter | Value |
|---|---|
| Detection Range | 0.312–20 ng/mL |
| Sensitivity | 0.172 ng/mL |
| Intra-/Inter-Assay CV | <10% (provided with kit) |
| Sample Types | Serum, plasma, tissue homogenates |
This kit employs a sandwich ELISA format, leveraging antibodies specific to the chicken FZD1 CRD .
Wnt Signaling Studies
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Cardiac Hypertrophy: Recombinant FZD1 immunization in mice attenuated post-infarct hypertrophy by blocking Wnt/β-catenin signaling, reducing heart weight by 22% and improving ejection fraction .
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Cancer: FZD1 overexpression correlates with drug resistance in acute myeloid leukemia (AML) and pancreatic ductal adenocarcinoma (PDAC). Silencing FZD1 reversed chemoresistance in NSCLC cells .
Developmental Biology
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Embryogenesis: CRISPR-mediated FZD1 knockdown in chicken models disrupted axis formation and somite patterning, confirming its role in Wnt/planar cell polarity pathways .
Mechanistic Insights
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Autoimmunization Strategy: Subcutaneous injection of recombinant FZD1 in mice generated anti-FZD1 antibodies that bound transmembrane FZD1, inhibiting Wnt signaling and hypertrophy .
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Cross-Species Reactivity: Chicken FZD1 shares 97% sequence identity with bovine and 94% with human isoforms, enabling translational studies .
Challenges and Future Directions
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them when placing your order. We will accommodate your request if possible.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance. Additional fees will apply.
Generally, liquid form has a shelf life of 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
