Recombinant Chicken Frizzled-6 (FZD6)
Description
Introduction
Frizzled-6 (FZD6) is a member of the Frizzled family of proteins, which serve as receptors in the Wnt signaling pathway . The Wnt pathway is crucial in regulating various developmental processes, including cell fate determination, tissue polarity, and organogenesis . Recombinant Chicken Frizzled-6 (FZD6) refers to the FZD6 protein produced using recombinant DNA technology in chicken cells, offering a valuable tool for studying its function and interactions.
Production and Characterization of Recombinant Chicken FZD6
Recombinant Chicken FZD6 is produced using various expression systems to ensure its availability for research purposes . The protein's molecular weight and purity are rigorously assessed to confirm its identity and quality . Such recombinant proteins are instrumental in conducting in vitro and in vivo studies to elucidate the specific roles of FZD6 in different biological contexts.
Role in Avian Biology
In avian species, FZD6 plays a crucial role during development. Studies involving chicken embryos have shown that FZD2, a related Frizzled receptor, influences craniofacial development and beak ossification . Although the research specifies FZD2, it suggests the importance of Frizzled proteins in avian developmental processes, potentially extending to FZD6 .
Involvement in Disease
Research indicates that FZD6 dysregulation is implicated in various diseases. Mutations in FZD6 have been associated with isolated autosomal-recessive nail dysplasia, characterized by claw-shaped nails, onychauxis, and onycholysis in humans . While this finding is in human models, it underscores the importance of FZD6 in tissue development and maintenance, suggesting potential implications in avian diseases as well.
Wnt Signaling and FZD6
FZD6 has the ability to interact with both WNT-3A and WNT-5A, recruiting β-catenin-dependent as well as β-catenin-independent pathways . Studies have shown that FZD6 mediates β-catenin-dependent signaling and that FZD6-null mutant cells have lost their capability to respond to WNT-3A stimulation . Furthermore, perturbed response has also been observed to β-catenin-independent WNT-5A stimulation in the absence of FZD6 .
Applications in Research
Recombinant Chicken FZD6 is used in diverse research applications:
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Signaling Studies: To investigate the specific signaling pathways activated by FZD6 and their downstream effects.
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Protein Interaction Assays: To identify proteins that interact with FZD6, providing insights into its functional mechanisms.
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Developmental Biology: To study the role of FZD6 in embryonic development and tissue formation.
Table: Comparison of Frizzled Receptors
Product Specs
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Target Background
Frizzled-6 (FZD6) is a receptor for Wnt proteins. Most FZD receptors are coupled to the canonical beta-catenin signaling pathway, leading to activation of Dishevelled proteins, inhibition of GSK-3 kinase, nuclear translocation of beta-catenin, and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been observed in some family members; however, its distinctness or integration with the canonical pathway remains unclear, as PKC appears necessary for Wnt-mediated GSK-3 kinase inactivation. Both pathways appear to involve G-protein interactions. Wnt5A activation stimulates PKC activity via a G-protein-dependent mechanism. FZD6 is involved in the transduction and intercellular transmission of polarity information during tissue morphogenesis and in differentiated tissues. In conjunction with FZD3, it may participate in neural tube closure and regulate the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric stereocilia bundles on the apical surfaces of specific auditory and vestibular sensory cells within the inner ear.
