Recombinant Chicken Methylsterol monooxygenase 1 (MSMO1)
Description
Introduction to Recombinant Chicken Methylsterol Monooxygenase 1 (MSMO1)
Recombinant Chicken Methylsterol Monooxygenase 1 (MSMO1) is a protein produced through recombinant DNA technology. It is a full-length protein derived from the chicken gene, expressed in Escherichia coli (E. coli) and tagged with a His-tag for purification and identification purposes . MSMO1, also known as sterol-C4-methyl oxidase (SC4MOL), plays a crucial role in the cholesterol biosynthesis pathway by catalyzing the demethylation of C4-methylsterols .
Characteristics of Recombinant Chicken MSMO1
The recombinant chicken MSMO1 protein is characterized by its full-length sequence of 296 amino acids, with an N-terminal His-tag. It is provided in a lyophilized powder form and has a purity of greater than 90% as determined by SDS-PAGE. The protein is stored in a Tris/PBS-based buffer with 6% trehalose at pH 8.0 .
| Characteristics | Description |
|---|---|
| Species | Chicken |
| Source | E. coli |
| Tag | His-tag |
| Protein Length | Full Length (1-296) |
| Form | Lyophilized powder |
| Purity | >90% (SDS-PAGE) |
| Storage Buffer | Tris/PBS-based buffer, 6% Trehalose, pH 8.0 |
Biological Function of MSMO1
MSMO1 is involved in the cholesterol biosynthesis pathway, specifically in the demethylation of C4-methylsterols. This enzyme is crucial for the progression of cholesterol synthesis, which is essential for various cellular processes, including membrane formation and hormone production .
Research Findings and Applications
While specific research on recombinant chicken MSMO1 is limited, studies on MSMO1 in general highlight its role in cancer progression and drug resistance. In cervical squamous cell carcinoma, high MSMO1 expression is associated with poor prognosis and may serve as a prognostic marker . The recombinant protein could potentially be used in research related to cholesterol metabolism and cancer biology.
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized fulfillment.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The specific tag type is determined during production. To request a particular tag, please specify it in your order; we will prioritize fulfilling such requests.
Target Background
Catalyzes the initial step in the removal of the two C-4 methyl groups from 4,4-dimethylzymosterol.
