Recombinant Chicken Peripherin-2 (PRPH2)
Description
Production Methods
Recombinant Chicken PRPH2 is synthesized using heterologous expression systems, including E. coli, yeast, baculovirus, or mammalian cells . The protein is purified to ≥85% purity via SDS-PAGE .
| Host Systems | Purity | Applications |
|---|---|---|
| E. coli | ≥85% | Structural studies, bioassays |
| Baculovirus | ≥85% | Functional assays, protein complexes |
| Mammalian Cells | ≥85% | Post-translational modification studies |
3.1. Gene Therapy and Disease Modeling
PRPH2 overexpression in Rom1−/− mice rescues OS disc enclosure defects, demonstrating its redundancy with ROM1 in OS morphogenesis . Overexpression of PRPH2 (30% above wild-type levels) restores normal disc structure in Rom1−/− retinas .
3.2. Antibody Development
Chicken polyclonal antibodies against PRPH2 are used in:
-
Western Blot (WB): 1:20,000 dilution to detect PRPH2 in rat retina/eye tissue .
-
Immunofluorescence (IF/ICC): 1:2,000–5,000 dilution for localization studies .
| Antibody Characteristics | Details |
|---|---|
| Immunogen | Recombinant rat PRPH2 (E. coli) |
| Cross-Reactivity | Human, Rat, Mouse, Pig, Cow |
| Molecular Weight | ~57 kDa (predicted), ~35–39 kDa (observed) |
4.1. PRPH2-ROM1 Interactions
PRPH2 forms hetero-oligomers with ROM1, which are essential for OS stability. In Rom1−/− mice, PRPH2 overexpression compensates for ROM1 loss, restoring disc enclosure .
| Condition | PRPH2:ROM1 Ratio | Outcome |
|---|---|---|
| Wild-Type (WT) | 2:1 | Normal OS structure |
| Rom1−/− | N/A | Disrupted discs (rescued by PRPH2 OE) |
4.2. Disease Mechanisms
Pathogenic PRPH2 mutations (e.g., R172W) disrupt complex formation with ROM1, causing aggregation and OS degeneration . Overexpression of wild-type PRPH2 in rds+/− mice improves rod function but not cone defects, highlighting species-specific vulnerabilities .
Therapeutic Implications
PRPH2 is a target for gene therapy in retinal degenerations. AAV-mediated delivery of PRPH2 improves OS structure in rod-dominant models (e.g., rds+/− mice), though cone-specific challenges remain .
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested in advance. Additional fees apply for dry ice shipping.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
- Our study reveals contrasting roles of per(WT) and Rom-1 in rod outer segment (OS) targeting of adRP-linked peripherin-2 mutants, suggesting a novel therapeutic strategy for affected individuals. PMID: 28539581
