Recombinant Chicken Protein-tyrosine sulfotransferase 2 (TPST2), partial
Description
Introduction to Recombinant Chicken Protein-tyrosine sulfotransferase 2 (TPST2), partial
Recombinant Chicken Protein-tyrosine sulfotransferase 2 (TPST2), partial, is a recombinant form of the enzyme tyrosylprotein sulfotransferase 2, specifically produced in yeast. This enzyme plays a crucial role in the post-translational modification of proteins by sulfating tyrosine residues, which is essential for various cellular processes, including protein-protein interactions and signal transduction pathways .
Background on Tyrosine Sulfation
Tyrosine sulfation is a post-translational modification that adds a sulfate group to tyrosine residues on proteins. This modification is significant in multicellular eukaryotes and is involved in enhancing the biological activity of proteins by altering their interactions with other molecules . Tyrosylprotein sulfotransferases (TPSTs) are the enzymes responsible for this modification, with TPST2 being one of the two main isoforms in mammals and other vertebrates .
Characteristics of Recombinant Chicken TPST2
The recombinant chicken TPST2 is produced in yeast, which offers several advantages, including high purity and cost-effectiveness . This form of TPST2 is partial, meaning it may lack certain regions compared to the full-length enzyme. Despite this, it retains the essential catalytic activity necessary for tyrosine sulfation.
Table 1: Key Features of Recombinant Chicken TPST2
| Feature | Description |
|---|---|
| Production Host | Yeast |
| Purity | High |
| Form | Partial |
| Function | Tyrosine sulfation |
Research Findings and Applications
Research on TPST2 has shown its critical role in specific biological pathways. For instance, TPST2 is essential for the efficient mediation of the TSH-TSHR signal transduction pathway . Additionally, tyrosine sulfation can enhance the binding affinity of antibodies by introducing negatively charged groups, which can improve their therapeutic efficacy .
Table 2: Biological Roles and Applications of TPST2
| Biological Role/Application | Description |
|---|---|
| Signal Transduction | Essential for TSH-TSHR pathway |
| Antibody Binding | Enhances binding affinity through tyrosine sulfation |
| Protein-Protein Interactions | Modulates interactions by altering protein surface properties |
References
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Tyrosine Sulfation as a Protein Post-Translational Modification - PMC
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Genetically Encoded Protein Sulfation in Mammalian Cells - PMC
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Recombinant Chicken Protein-tyrosine sulfotransferase 2 (TPST2) - Cusabio
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A Mutation in Tpst2 Encoding Tyrosylprotein Sulfotransferase
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Tyrosine Sulfation at Antibody Light Chain CDR-1 Increases Binding
Product Specs
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Target Background
Function: Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, utilizing 3'-phosphoadenylyl sulfate (PAPS) as a cosubstrate.
